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patA

N-acetyl-L,L-diaminopimelate aminotransferase

locus: BSU_14000

pI: 6.01

mw: 43.29 kDa

protein length: 393 aa

gene length: 1182 bp

function: biosynthesis of lysine and peptidoglycan

product: aminotransferase

essential: yes

ec: 2.6.1.-

synonyms: uat,dapX

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.6|Biosynthesis/ acquisition of lysine/ threonine]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 1,471,857 1,473,038

Phenotypes of a mutant

• essential [Pubmed|23109554]

The protein

Catalyzed reaction/ biological activity

• N-acetyl-2-amino-6-ketopimelate - N-acetyl-L,L-2,6-diaminopimelate [Pubmed|24261876]
• 2-oxoglutarate + N-acetyl-LL-2,6-diaminoheptanedioate --> L-2-acetamido-6-oxoheptanedioate + L-glutamate (according to UniProt)

Protein family

• [SW|class-I pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

Paralogous protein(s)

• [protein|62A9CD9AF72D0A48EC5ABC1585E2D8636D36D021|AlaT], [protein|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|AspB]

[SW|Cofactors]

• PLP

Structure

• [PDB|1GDE] (from Pyrococcus sp., 42% identity) [pubmed|11134972]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|094AE3AC8DFAFC8048ADAAB34F76DF2562D80CE7|patA]
  description: [Pubmed|22383849]

• genes: [gene|5B177CBC33203B5B2DA841097CEB29B3A3C37C08|ykzT]-[gene|094AE3AC8DFAFC8048ADAAB34F76DF2562D80CE7|patA]
  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE14000 ([gene|094AE3AC8DFAFC8048ADAAB34F76DF2562D80CE7|patA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATTCATCACCTTAACA, downstream forward: _UP4_AACAACGGCGTTTAAGCCGT

References

• 24261876,23109554,11134972,29280348