iolB [Tue Dec 13 2016 11:16:37 GMT+0100 (CET)]

formation of 2-deoxy-5-keto-gluconic acid (4th reaction)

locus: BSU39750

pI: 4.77

mw: 30.00 kDa

function: myo-inositol catabolism

product: formation of 2-deoxy-5-keto-gluconic acid (4th reaction)

essential: no

synonyms: yxdB

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.16|Utilization of inositol]

[SW|Categories] containing this gene/protein

• [SW|utilization of specific carbon sources]

This gene is a member of the following [SW|regulons]

• [SW|CcpA regulon], [SW|IolR regulon]

Gene

Coordinates on the chromosome (coding sequence): 4,082,030 -> 4,082,845

The protein

Catalyzed reaction/ biological activity

• 5-deoxy-glucuronic acid = 5-dehydro-2-deoxy-D-gluconic acid (according to Swiss-Prot)

Protein family

• isomerase iolB family (according to Swiss-Prot)

Structure

• [PDB|2QJV] (from ''Salmonella typhimurium'', 49% identity, 65% similarity)

Expression and Regulation

Operon

• ''[protein|search|iolA]-[protein|search|iolB]-[protein|search|iolC]-[protein|search|iolD]-[protein|search|iolE]-[protein|search|iolF]-[protein|search|iolG]-[protein|search|iolH]-[protein|search|iolI]-[protein|search|iolJ]'' [Pubmed|9226270]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|9226270]

Regulation

• repressed by glucose ([protein|search|CcpA]) [Pubmed|10666464]
• induced by inositol ([protein|search|IolR]) [Pubmed|9887260,9226270]

Regulatory mechanism

• [protein|search|IolR]: transcription repression [Pubmed|9887260,9226270]
• [protein|search|CcpA]: transcription repression [Pubmed|10666464]

Labs working on this gene/protein

• [SW|Yasutaro Fujita], University of Fukuyama, Japan
• [[Ken-ichi Yoshida]], Kobe University, Japan

References

• 9226270,11566986,18310071,9887260,18310071