ispH

(E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 7th step in the MEP pathway of isoprenoid biosynthesis

locus: BSU_25160

pI: 5.61

mw: 34.77 kDa

protein length: 314 aa

gene length: 945 bp

function: MEP pathway of isoprenoid biosynthesis

product: (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase

essential: yes

ec: 1.17.1.2

synonyms: yqfP

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.3|Biosynthesis of isoprenoids]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 2,596,535 2,597,479

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• H₂O + isopentenyl diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] --> (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H⁺ + 2 reduced [2Fe-2S]-[ferredoxin] (according to UniProt)
• dimethylallyl diphosphate + H₂O + 2 oxidized [2Fe-2S]-[ferredoxin] --> (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H⁺ + 2 reduced [2Fe-2S]-[ferredoxin] (according to UniProt)

Protein family

• ispH family (single member, according to UniProt)

[SW|Cofactors]

• Fe-S cluster [pubmed|29292548]

Structure

• [PDB|3F7T] (IspH from ''E. coli'', 38% identity) [Pubmed|19569147]

Expression and Regulation

Operons

• genes: [gene|9B1EA9872500280D6360EECD3D7344A1974910CA|yqfQ]-[gene|08F1D9AD2D51D8513AFFA57E8ED69DB8EF97AD88|ispH]
  description: [Pubmed|22383849]

• genes: [gene|08F1D9AD2D51D8513AFFA57E8ED69DB8EF97AD88|ispH]
  description: [Pubmed|22383849]

References

• 17458547,19569147,23840410,27791134