competence transcription factor (CTF)
function
regulation of competence and DNA uptake
product
competence transcription factor (CTF)
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue][SW|Categories] containing this gene/protein
[SW|genetic competence], [SW|transcription factors and their control], [SW|phosphoproteins]This gene is a member of the following [SW|regulons]
[SW|AbrB regulon], [SW|CodY regulon], [SW|ComK regulon], [SW|DegU regulon], [SW|Rok regulon], [SW|Spo0A regulon]Gene
Coordinates on the chromosome (coding sequence)
1,117,109 -> 1,117,687
Phenotypes of a mutant
no expression of competence genes, loss of [SW|genetic competence] [Pubmed|7783616]The protein
Modification
phosphorylated on Arg-65, Arg-157, Arg-161, Arg-165, Arg-186, and Arg-191 [Pubmed|22517742][SW|Interactions]
[protein|search|MecA]-[protein|search|ComK] [Pubmed|10447896]Expression and Regulation
Operon
''comK'' [Pubmed|8196543][SW|Sigma factor]
[protein|search|SigA] [Pubmed|8196543]Regulation
''[SW|comK]'' has a bistable expression pattern [Pubmed|26110430]repressed by casamino acids [Pubmed|12107147]repressed by [protein|search|Rok] [Pubmed|11849533]repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|8830686]repressed during logarithmic growth ([protein|search|AbrB]) [Pubmed|12586407]induced at the entry to stationary phase ([SW|Spo0A]-P) [Pubmed|22412392]Regulatory mechanism
[protein|search|Rok]: transcription repression [Pubmed|11849533][protein|search|CodY]: transcription repression [Pubmed|8830686][protein|search|AbrB]: transcription repression [Pubmed|12586407][protein|search|ComK]: transcription activation (autoregulation) [Pubmed|8083168][protein|search|DegU] (non-phosphorylated form): transcription activation [Pubmed|8083168][SW|Spo0A]-P: transcription activation, acts as antagonist of [protein|search|Rok] [Pubmed|22412392]Additional information
degraded by [protein|search|ClpC]-[protein|search|ClpP] (with [protein|search|MecA] as adaptor protein) [PubMed|9890793]half-life of the ''[SW|comK]'' mRNA: 3.9 min, stabilized to 5.4 min in a ''[SW|kre]'' mutant [Pubmed|26110430]Biological materials
Mutant
1A871 (no resistance), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A871&Search=1A871 BGSC]Expression vector
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth labLabs working on this gene/protein
[SW|Oscar Kuipers], University of Groningen, The Netherlands[http://molgen.biol.rug.nl/molgen/index.php Homepage]References
Reviews
19995980,12576575,26110607 The [SW|ComK regulon]
Original Publications
8878039,11849533,17569828,9335269,17560370,12586407,11814663,9108277,9890793,9585513,15819619,10908654,9000055,7746142,8830686,10447896,12028382,8016067,10361283,11703662,12107147,17581123,8016066,17493123,16391071,16436435,12270822,15598897,20300532,12761164,12036071,21085679,22517742,7783616,22412392,8083168,24838881,8196543,26110430,27045827 
