lnrM

similar to [SW|ABC transporter] (membrane protein), involved in resistance to linearmycin

locus: BSU_08320

pI: 9.62

mw: 42.95 kDa

protein length: 396 aa

gene length: 1191 bp

function: resistance to linearmycin

product: [SW|ABC transporter] (membrane protein)

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.2|Exporters] → [category|SW 1.2.1.2.1|Efflux of antibiotics]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 906,764 907,954

Phenotypes of a mutant

• overexpression of [gene|07858BFC72EB0B6AF615C8D4C8BCC162D8C3E7D2|lnrM] and [gene|79BAF2329209C81E8D165F131F9DAADB4A81448A|lnrN] stimulates [SW|biofilm formation ][pubmed|28461449]

The protein

Protein family

• [SW|ABC-2 integral membrane protein family] (according to UniProt)

[SW|Domains]

• [SW|ABC transmembrane type-2 domain] (aa 161-396) (according to UniProt)

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|329E4DF9EF1545AABE485B9BBCD22F09ABF019A1|lnrL]-[gene|07858BFC72EB0B6AF615C8D4C8BCC162D8C3E7D2|lnrM]-[gene|79BAF2329209C81E8D165F131F9DAADB4A81448A|lnrN]
  description: [Pubmed|26647299]

  sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

  regulatory mechanism

  • [protein|387EF370CE24F7A3C20789A57329A02EBED46F53|LnrK]: activation, [Pubmed|26647299], in [regulon|387EF370CE24F7A3C20789A57329A02EBED46F53|LnrK regulon]

  regulation

  • expression during spore [SW|germination] is increased under conditions of osmotic stress [Pubmed|27766092]
  • induced in the presence of linearmycin and other polyenes ([protein|387EF370CE24F7A3C20789A57329A02EBED46F53|LnrK]) [pubmed|32973732,28461449]

Biological materials

Mutant

• MGNA-C302 (yfiM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2300 NBRP B. subtilis, Japan]
• BKE08320 ([gene|07858BFC72EB0B6AF615C8D4C8BCC162D8C3E7D2|lnrM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCTTTTTTTCATTGCCGTCC, downstream forward: _UP4_CATACAAGATAAGGAGGTAC
• BKK08320 ([gene|07858BFC72EB0B6AF615C8D4C8BCC162D8C3E7D2|lnrM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCTTTTTTTCATTGCCGTCC, downstream forward: _UP4_CATACAAGATAAGGAGGTAC

References

• 10092453,26647299,28461449