walH

negative effector of WalK, controls cell wall metabolism

Locus: BSU_40390

Isoelectric point: 5.59

Molecular weight: 52.05 kDa

Protein length: 455 aa

Gene length: 1368 bp

Function: control of cell wall metabolism

Product: negative effector of WalK

Essential: no

Synonyms: yycH

Genomic Context

Loading

Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall/ other
• Information processing → Regulation of gene expression → Transcription factors and their control → Control of two-component response regulators → Control of response regulators/ other
• Groups of genes → Membrane proteins

Gene

Coordinates: 4,150,496 4,151,863

Phenotypes of a mutant

• growth defect at high temperature, lysis at 49C, this can be suppressed by mutations that reduce WalK autokinase activity, by inactivation of lytE, or by overexpreesion of IseA or PdaC PubMed

The protein

Structure

• 2FGT

Localization

• cell membrane (according to UniProt), spotty close to the membrane PubMed

Expression and Regulation

Operons

• Genes: walR-walK-walH-walI-walJ-htrC
  Description: PubMed

  Regulation

  • htrC transcript expressed during sporulation PubMed

Biological materials

Mutant

• MGNA-B826 (yycH::erm), available at the NBRP B. subtilis, Japan
• BKE40390 (walH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CGTAAGTAATATCGTTTTTA, downstream forward: _UP4_TTATTGAGGAAGGAGGGGGC
• BKK40390 (walH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CGTAAGTAATATCGTTTTTA, downstream forward: _UP4_TTATTGAGGAAGGAGGGGGC

References

Reviews

Loading

Original publications

Loading