fmt [2017-8-11 16:0:3]

methionyl-tRNA formyltransferase

Locus: BSU15730

Isoelectric point: 5.62

Molecular weight: 34.48 kDa

Protein length: 317 aa

Gene length: 951 bp

Function: formylation of Met-tRNA(fMet)

Product: methionyl-tRNA formyltransferase

Essential: no

E.C.: 2.1.2.9

Synonyms: yloL

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation
• Groups of genes → Efp-dependent proteins

Gene

Coordinates: 1,646,999 → 1,647,952

Phenotypes of a mutant

• inactivation of fmt suppresses the synthetic lethality of the defA defB double mutant PubMed
• essential according to Kobayashi et al. PubMed, but a deletion mutant was constructed by Cai et al. PubMed
• the mutant is defective for biofilm formation, motility, and sporulation PubMed
• the mutant exhibits increased sensitivity to metal ion excess and oxidative stress PubMed

The protein

Catalyzed reaction/ biological activity

• 10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) + H₂O = tetrahydrofolate + N-formylmethionyl-tRNA(fMet) (according to Swiss-Prot)

Protein family

• fmt family (according to Swiss-Prot)

Structure

• 3RFO (from Bacillus anthracis, 69% identity, 89% similarity)

Expression and Regulation

Operons

• Genes: defA-fmt-yloM-yloN
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

• Genes: yloC-remA-gmk-yloH-yloI-priA-defA-fmt-yloM-yloN

  Description

  • PubMed

Additional information

• translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

References

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