ptsA

PTS EIIA component

Locus: BSU_22230

Isoelectric point: 5.29

Molecular weight: 17.79 kDa

Protein length: 168 aa

Gene length: 507 bp

Function: uptake and phosphorylation of maltose, N-acetylglucosamine, sucrose, and trehalose

Product: PTS EIIA component

Essential: no

Synonyms: ypqE

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Phosphotransferase system → Sugar specific PTS proteins
• Groups of genes → Phosphoproteins → Phosphorylation on a His residue

Gene

Coordinates: 2,336,933 2,337,439

The protein

Protein family

• PTS permease, glucose family PubMed

Domains

• PTS EIIA domain type-1 (aa 36-140) (according to UniProt)

Structure

• 1AX3 (IIA domain of PtsG, 46% identity) PubMed

Expression and Regulation

Operons

• Genes: ptsA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

Biological materials

Mutant

• MGNA-A477 (ypqE::erm), available at the NBRP B. subtilis, Japan
• QB6099 (aphA3), available in Jörg Stülke's lab PubMed
• BKE22230 (ypqE::ermC) (available at the BGSC and in Jörg Stülke's lab) PubMed
• GP1863 (ypqE::ermC) (available in Jörg Stülke's lab)
• BKE22230 (ptsA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATGTATTTTCTCCCTTCT, downstream forward: _UP4_TAAGCAGGGCGTATGCCTTG
• BKK22230 (ptsA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATGTATTTTCTCCCTTCT, downstream forward: _UP4_TAAGCAGGGCGTATGCCTTG

References

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