SubtiBank SubtiBank
yfjO [2019-08-16 10:33:09]
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yfjO [2019-08-16 10:33:09]

similar to ribosomal RNA methyltransferase
locus
BSU_08020
pI
9.81
mw
51.97 kDa
protein length
466 aa Sequence Blast
gene length
1401 bp Sequence Blast
function
unknown
product
putative rRNA methyltransferase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.3|rRNA modification and maturation/ based on similarity]

    • Gene

    Coordinates
    873,402 874,802

    The protein

    Protein family

  • [SW|Methyltransferase superfamily] (according to UniProt)
  • [SW|class I-like SAM-binding methyltransferase superfamily] (according to UniProt)

    • Paralogous protein(s)

  • [protein|B97ABE3233A27A7C8273ED9A1E688247DD37FD12|RlmCD]

    • [SW|Cofactors]

  • Fe-S cluster [pubmed|29292548]

    • Structure

  • [PDB|5XJ1] (from Streptococcus pneumoniae, 35% identity) [pubmed|28949991]

    • Expression and Regulation

    Operons

    genes
    [gene|82CDC97D16426016CA7E75240C1A6D0D600F4375|yfjP]-[gene|05392BB2C32B0405799D4F34E5BA55FC936AB943|yfjO]
    description
    [pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-C276 (yfjO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2274 NBRP B. subtilis, Japan]
  • BKE08020 ([gene|05392BB2C32B0405799D4F34E5BA55FC936AB943|yfjO]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTGTTCTCTCCGTTTC, downstream forward: _UP4_TAAAAAGAACATTTCCCAGT
  • BKK08020 ([gene|05392BB2C32B0405799D4F34E5BA55FC936AB943|yfjO]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTGTTCTCTCCGTTTC, downstream forward: _UP4_TAAAAAGAACATTTCCCAGT

    • References

  • 21824914,28949991