yfjO [2019-07-01 09:26:01]

similar to ribosomal RNA methyltransferase

locus: BSU08020

pI: 9.81

mw: 51.97 kDa

protein length: 466 aa

gene length: 1401 bp

function: unknown

product: putative rRNA methyltransferase

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.3|rRNA modification and maturation/ based on similarity]

Gene

Coordinates: 873,402 874,802

The protein

Protein family

• [SW|Methyltransferase superfamily] (according to UniProt)

Paralogous protein(s)

• [protein|B97ABE3233A27A7C8273ED9A1E688247DD37FD12|RlmCD]

[SW|Cofactors]

• Fe-S cluster [pubmed|29292548]

Structure

• [PDB|5XJ1] (from Streptococcus pneumoniae, 35% identity) [pubmed|28949991]

Expression and Regulation

Operons

• genes: [gene|82CDC97D16426016CA7E75240C1A6D0D600F4375|yfjP]-[gene|05392BB2C32B0405799D4F34E5BA55FC936AB943|yfjO]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C276 (yfjO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2274 NBRP B. subtilis, Japan]
• BKE08020 ([gene|05392BB2C32B0405799D4F34E5BA55FC936AB943|yfjO]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTGTTCTCTCCGTTTC, downstream forward: _UP4_TAAAAAGAACATTTCCCAGT
• BKK08020 ([gene|05392BB2C32B0405799D4F34E5BA55FC936AB943|yfjO]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTGTTCTCTCCGTTTC, downstream forward: _UP4_TAAAAAGAACATTTCCCAGT

References

• 21824914,28949991