SubtiBank SubtiBank
comFC
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comFC

late competence gene
Locus
BSU_35450
Isoelectric point
8.85
Molecular weight
26.26 kDa
Protein length
229 aa Sequence Blast
Gene length
690 bp Sequence Blast
Function
compentence
Product
unknown
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,641,125 3,641,814

The protein

Protein family

  • comF/gntX family (single member, according to UniProt)
  • Modification

  • phosphorylated on Arg-74 PubMed
  • Localization

  • localizes to one or both cell poles PubMed
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigD: sigma factor, PubMed, in SigD regulon
  • Regulatory mechanism

  • ComK: activation, PubMed, in ComK regulon
  • DegU: activation, DegU-P, PubMed, in DegU regulon
  • ScoC: repression, PubMed, in ScoC regulon
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    Biological materials

    Mutant

  • BKE35450 (comFC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTGCGAATCACATAATAAAC, downstream forward: _UP4_TAAATGATTCTGTTTTTATG
  • BKK35450 (comFC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTGCGAATCACATAATAAAC, downstream forward: _UP4_TAAATGATTCTGTTTTTATG
  • Expression vectors

  • for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
  • References

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