metQ [2019-06-26 09:11:52]

methionine [SW|ABC transporter] (binding lipoprotein)

locus: BSU32730

pI: 8.49

mw: 30.20 kDa

protein length: 274 aa

gene length: 825 bp

function: methionine uptake

product: methionine [SW|ABC transporter] (binding lipoprotein)

essential: no

synonyms: yusA

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.2|Uptake of amino acids]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.10|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,361,767 3,362,591

The protein

Protein family

• nlpA lipoprotein family (with [protein|0481A9C134A6EEC6F111AD0C03E2E345D6A315A0|MetQ], according to UniProt)

Paralogous protein(s)

• [protein|9BFC99C85F7BEA5FEA6B8E82D205EED02375240E|YhcJ]

Structure

• [PDB|4GOT]

[SW|Localization]

• associated to the cell membrane, via [protein|F94070DD039A422055FFC35087FB87765F22E156|MetP] [Pubmed|10092453,18763711]
• extracellular (signal peptide) [Pubmed|18957862], lipid modification as retention signal

Expression and Regulation

Operons

• genes: [gene|D7254B7F31D3F4CD18172AFB0F526CAE5195BDB6|metN]-[gene|F94070DD039A422055FFC35087FB87765F22E156|metP]-[gene|0481A9C134A6EEC6F111AD0C03E2E345D6A315A0|metQ]
  description: [Pubmed|10094622]

  regulatory mechanism

  • [regulon|S-box|S-box]: transcription termination/ antitermination, the [SW|S-box] [SW|riboswitch] binds S-adenosylmethionine resulting in termination, in [regulon|S-box|S-box]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: activation, [Pubmed|12618455], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

  regulation

  • activated during growth in the presence of branched chain amino acids ([protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]) [Pubmed|12618455]
  • the mRNA is processed between [gene|F94070DD039A422055FFC35087FB87765F22E156|metP] and [gene|0481A9C134A6EEC6F111AD0C03E2E345D6A315A0|metQ] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]
  • the [SW|S-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

Biological materials

Mutant

• MGNA-A595 (yusA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/595 NBRP B. subtilis, Japan]
• BKE32730 ([gene|0481A9C134A6EEC6F111AD0C03E2E345D6A315A0|metQ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAGCTTTTTCAATGTAAATC, downstream forward: _UP4_TAAGACTGAAACCCCGGATG
• BKK32730 ([gene|0481A9C134A6EEC6F111AD0C03E2E345D6A315A0|metQ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAGCTTTTTCAATGTAAATC, downstream forward: _UP4_TAAGACTGAAACCCCGGATG

References

• 10092453,14990259,12618455,18957862,18763711,10094622,18039762,23038252,29794222