bglS [2017-06-23 17:43:30]

endo-beta-1,3-1,4 glucanase

locus: BSU39070

pI: 6.48

mw: 27.12 kDa

protein length: 242 aa

gene length: 726 bp

function: lichenan degradation

product: endo-beta-1,3-1,4 glucanase

essential: no

ec: 3.2.1.73

synonyms: bgl,licS

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.27|Utilization of other polymeric carbohydrates]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 4,011,842 → 4,012,570

The protein

Catalyzed reaction/ biological activity

• Hydrolysis of (1→4)-beta-D-glucosidic linkages in beta-D-glucans containing (1→3)- and (1→4)-bonds (according to Swiss-Prot)

Protein family

• glycosyl hydrolase 16 family (according to Swiss-Prot)

Structure

• [PDB|3O5S]

[SW|Localization]

• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|044A0EBC8798B110E719B73664EB80F53112D8DE|bglS]
  description: [Pubmed|8606172]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8606172], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|8245831], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|LicT]: antitermination, via binding to an [SW|RNA switch], in [regulon|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|LicT regulon]

  regulation

  • expressed in the stationary phase (temporal activation) [Pubmed|8245830]

• genes: [gene|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|licT]-[gene|044A0EBC8798B110E719B73664EB80F53112D8DE|bglS]
  description: [Pubmed|8606172]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8606172], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulation

  • expressed in the stationary phase (temporal activation) [Pubmed|8245830]

• genes: [gene|6BC5A936D8EDE7087C3F6293A2873EC5352FE5EF|yxiP]-[gene|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|licT]-[gene|044A0EBC8798B110E719B73664EB80F53112D8DE|bglS]
  description: [Pubmed|22383849]

Biological materials

Mutant

• GP427 (''[gene|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|licT]-[gene|044A0EBC8798B110E719B73664EB80F53112D8DE|bglS]'', erm), BGW7 (cat), both available in the [SW|Stülke] lab

References

• 18957862,12850135,6087283,8245830,8245831,8606172,23459129,24391357,24659048,25808979,28084659,28235843