bglS

endo-beta-1,3-1,4 glucanase

locus: BSU_39070

pI: 6.48

mw: 27.12 kDa

protein length: 242 aa

gene length: 729 bp

function: lichenan degradation

product: endo-beta-1,3-1,4 glucanase

essential: no

ec: 3.2.1.73

synonyms: bgl,licS

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.27|Utilization of other polymeric carbohydrates]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 4,011,842 4,012,570

The protein

Catalyzed reaction/ biological activity

• Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds (according to UniProt)

Protein family

• glycosyl hydrolase 16 family (single member, according to UniProt)

[SW|Domains]

• GH16 domain (aa 29-242) (according to UniProt)

Structure

• [PDB|3O5S]

[SW|Localization]

• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|044A0EBC8798B110E719B73664EB80F53112D8DE|bglS]
  description: [Pubmed|8606172]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8606172], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|8245831], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|LicT]: antitermination, via binding to an [SW|RNA switch], in [regulon|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|LicT regulon]

  regulation

  • expressed in the stationary phase (temporal activation) [Pubmed|8245830]

• genes: [gene|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|licT]-[gene|044A0EBC8798B110E719B73664EB80F53112D8DE|bglS]
  description: [Pubmed|8606172]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8606172], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulation

  • expressed in the stationary phase (temporal activation) [Pubmed|8245830]

• genes: [gene|6BC5A936D8EDE7087C3F6293A2873EC5352FE5EF|yxiP]-[gene|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|licT]-[gene|044A0EBC8798B110E719B73664EB80F53112D8DE|bglS]
  description: [Pubmed|22383849]

Biological materials

Mutant

• GP427 ([gene|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|licT]-[gene|044A0EBC8798B110E719B73664EB80F53112D8DE|bglS]::erm), BGW7 (cat), both available in [SW|Jörg Stülke]'s lab
• BKE39070 ([gene|044A0EBC8798B110E719B73664EB80F53112D8DE|bglS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGGCATTCCCCTTTC, downstream forward: _UP4_TAATGCCAAATGTGAAAGAG
• BKK39070 ([gene|044A0EBC8798B110E719B73664EB80F53112D8DE|bglS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGGCATTCCCCTTTC, downstream forward: _UP4_TAATGCCAAATGTGAAAGAG

References

• 18957862,12850135,6087283,8245830,8245831,8606172,23459129,24391357,24659048,25808979,28084659,28235843,30733736