speA

arginine decarboxylase, required for biofilm formation

Locus: BSU_14630

Isoelectric point: 5.08

Molecular weight: 53.42 kDa

Protein length: 490 aa

Gene length: 1470 bp

Function: spermidine, polyamine biosynthesis

Product: arginine decarboxylase

Essential: no

E.C.: 4.1.1.19

Synonyms: cad

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Miscellaneous metabolic pathways → Metabolism of polyamines
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Other proteins required for biofilm formation

Gene

Coordinates: 1,534,279 → 1,535,751

Phenotypes of a mutant

• no biofilm formation PubMed

The protein

Catalyzed reaction/ biological activity

• H⁺ + L-arginine --> agmatine + CO₂ (according to UniProt)

Protein family

• Orn/Lys/Arg decarboxylase class-I family (together with YaaO) (according to UniProt)

Paralogous protein(s)

• YaaO

Cofactors

• PLP (according to UniProt)

Structure

• 2X3L (from Staphylococcus aureus, 28% identity) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: speA
  Description: PubMed

Biological materials

Mutant

• CS210 (speA::aphA3, available in Colin Harwood's and Jörg Stülke's labs) PubMed
• BKE14630 (ΔspeA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTGTTTTCCCACCTTTG, downstream forward: _UP4_TAAAAAATAAAAAGCATGCG
• BKK14630 (ΔspeA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTGTTTTCCCACCTTTG, downstream forward: _UP4_TAAAAAATAAAAAGCATGCG

References

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