dinG

ATP-dependent DNA helicase

locus: BSU_22400

pI: 5.82

mw: 105.83 kDa

protein length: 931 aa

gene length: 2796 bp

function: response to DNA damage

product: ATP-dependent DNA helicase

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]

Gene

Coordinates: 2,349,671 2,352,466

The protein

Protein family

• [SW|helicase family] (according to UniProt)

Paralogous protein(s)

• [protein|0F9B77CE6C662D2F249C7E33E5C553A74C390259|YpvA]

[SW|Domains]

• [SW|Exonuclease domain] (aa 7-162) (according to UniProt)
• [SW|Helicase ATP-binding domain] (aa 250-510) (according to UniProt)
• [SW|Helicase C-terminal domain] (aa 741-897) (according to UniProt)

Structure

• [PDB|4A15] (from Thermoplasma acidophilum, corresponds to aa 257 ... 913 of DinG, 22% identity) [pubmed|22081108]

[SW|Localization]

• Cytoplasm (Homogeneous) [Pubmed|16479537]

Biological materials

Mutant

• MGNA-A423 (dinG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/423 NBRP B. subtilis, Japan]
• BKE22400 ([gene|03BFD8AD2E3D95858752F46366F6C2028D709029|dinG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAAAATGGACACCTCAA, downstream forward: _UP4_TAAAAGCCGCATAAAGCGGC
• BKK22400 ([gene|03BFD8AD2E3D95858752F46366F6C2028D709029|dinG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22400 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CATTAAAATGGACACCTCAA, downstream forward: _UP4_TAAAAGCCGCATAAAGCGGC

References

• 16479537,22081108