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tgt
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tgt

tRNA-guanine transglycosylase
Locus
BSU_27710
Isoelectric point
6.15
Molecular weight
43.55 kDa
Protein length
381 aa Sequence Blast
Gene length
1146 bp Sequence Blast
Function
tRNA modification
Product
tRNA-guanine transglycosylase
Essential
no
E.C.
2.4.2.29
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation

    • Gene

    Coordinates
    2,832,727 2,833,872

    The protein

    Catalyzed reaction/ biological activity

  • 7-aminomethyl-7-carbaguanine + guanosine34 in tRNA --> 7-aminomethyl-7-carbaguanosine34 in tRNA + guanine (according to UniProt)

    • Protein family

  • queuine tRNA-ribosyltransferase family (single member, according to UniProt)

    • Structure

  • 1PUD (from Zymomonas mobilis, 52% identity) PubMed

    • Localization

  • cytosol(according to UniProt)

    • Expression and Regulation

    Operons

    Genes
    ruvA-ruvB-yrzS-queA-tgt-yrbF
    Description
    PubMed

    Regulation

  • the mRNA is processed between tgt and yrbF by RNase Y, this requires the YmcA-YlbF-YaaT complex PubMed
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    Biological materials

    Mutant

  • BKE27710 (tgt::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTTTTGATGAGGCCTCCT, downstream forward: _UP4_TAAATAAACATAACAGGCTT
  • BKK27710 (tgt::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTTTTGATGAGGCCTCCT, downstream forward: _UP4_TAAATAAACATAACAGGCTT

    • References

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