tgt

tRNA-guanine transglycosylase

locus: BSU_27710

pI: 6.15

mw: 43.55 kDa

protein length: 381 aa

gene length: 1146 bp

function: tRNA modification

product: tRNA-guanine transglycosylase

essential: no

ec: 2.4.2.29

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]

Gene

Coordinates: 2,832,727 2,833,872

The protein

Catalyzed reaction/ biological activity

• 7-aminomethyl-7-carbaguanine + guanosine³⁴ in tRNA --> 7-aminomethyl-7-carbaguanosine³⁴ in tRNA + guanine (according to UniProt)

Protein family

• queuine tRNA-ribosyltransferase family (single member, according to UniProt)

Structure

• [PDB|1PUD] (from Zymomonas mobilis, 52% identity) [pubmed|8654383]

[SW|Localization]

• cytosol(according to UniProt)

Expression and Regulation

Operons

• genes: [gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[gene|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]-[gene|6CDF1836F5B613A6718308A4A27D713193EA197B|yrzS]-[gene|23174B2A19DEDF2F20858A005D0681D36E15E6E3|queA]-[gene|0383C8214ADE2FF70CFDB953A65648B37A9D3216|tgt]-[gene|D15B74263167DAD3FA9B7384B5AA4897EAD498F2|yrbF]
  description: [pubmed|22383849]

  regulation

  • the mRNA is processed between [gene|0383C8214ADE2FF70CFDB953A65648B37A9D3216|tgt] and [gene|D15B74263167DAD3FA9B7384B5AA4897EAD498F2|yrbF] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]

Biological materials

Mutant

• BKE27710 ([gene|0383C8214ADE2FF70CFDB953A65648B37A9D3216|tgt]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTTGATGAGGCCTCCT, downstream forward: _UP4_TAAATAAACATAACAGGCTT
• BKK27710 ([gene|0383C8214ADE2FF70CFDB953A65648B37A9D3216|tgt]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTTGATGAGGCCTCCT, downstream forward: _UP4_TAAATAAACATAACAGGCTT

References

• 6804468,14523925,12949492,8494901,7698334,7893665,7615526,8608154,12877882,8654383,29794222