yfmL [Tue Dec 13 2016 11:16:31 GMT+0100 (CET)]

DEAD-box RNA helicase

locus: BSU07430

pI: 9.60

mw: 42.00 kDa

function: RNA helicase

product: DEAD-box RNA helicase

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.3|DEAD-box RNA helicases]
• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.6|Ribosome assembly]

[SW|Categories] containing this gene/protein

• [SW|DEAD-box RNA helicases], [SW|translation]

Gene

Coordinates on the chromosome (coding sequence): 816,113 -> 817,243

Phenotypes of a mutant

• poor growth at low temperatures (16 to 20°C) [Pubmed|23175651]

The protein

Catalyzed reaction/ biological activity

• RNA helicase

Protein family

• helicase C-terminal domain (according to Swiss-Prot) [DEAD-box RNA helicases|search|DEAD-box RNA helicase]

Paralogous protein(s)

• [protein|search|CshA], [protein|search|CshB], [protein|search|DeaD]

Expression and Regulation

Operon

• ''yfmL'' (according to [http://dbtbs.hgc.jp/COG/prom/yfmL.html DBTBS])

Regulation

• expression declines in the stationary phase [Pubmed|23175651]

Biological materials

Mutant

• GP1053 (erm), available in the [SW|Stülke] lab

Expression vector

• for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [SW|SPINE], expression from the native chromomsomal site: GP1069 (spc), available in [SW|Jörg Stülke]'s lab

GFP fusion

• B. subtilis GP1082 yfmL-yfp spc, available in [SW|Jörg Stülke]'s lab

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

FLAG-tag construct

• GP1066 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s

References

• 23175651