yfmL [2017-06-23 17:02:49]

DEAD-box RNA helicase

Locus: BSU07430

Isoelectric point: 9.60

Molecular weight: 42.03 kDa

Protein length: 376 aa

Gene length: 1128 bp

Function: RNA helicase

Product: DEAD-box RNA helicase

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → RNA synthesis and degradation → DEAD-box RNA helicases
• Information processing → Protein synthesis, modification and degradation → Translation → Ribosome assembly

Gene

Coordinates: 816,113 → 817,243

Phenotypes of a mutant

• poor growth at low temperatures (16 to 20°C) PubMed

The protein

Catalyzed reaction/ biological activity

• RNA helicase
• required for ribosome assembly PubMed

Protein family

• helicase C-terminal domain (according to Swiss-Prot) DEAD-box RNA helicases

Paralogous protein(s)

• CshA, CshB, DeaD

Structure

• 5IVL (CshA, 87% coverage, 35% identity) PubMed

Expression and Regulation

Operons

• Genes: yfmL
  Description: PubMed

  Regulation

  • expression declines in the stationary phase PubMed

Biological materials

Mutant

• MGNA-C239 (yfmL::erm), available at the NBRP B. subtilis, Japan
• GP1053 (erm), available in the Stülke lab

Expression vector

• for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1069 (spc), available in Jörg Stülke's lab

GFP fusion

• B. subtilis GP1082 yfmL-yfp spc, available in Jörg Stülke's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

FLAG-tag construct

• GP1066 (spc, based on pGP1331), available in Jörg Stülke's

References

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