sirA [Tue Dec 13 2016 11:16:33 GMT+0100 (CET)]

sporulation protein, inhibits DNA replication initiation in cells committed to sporulation

locus: BSU17900

pI: 7.00

mw: 17.00 kDa

function: control of chromosome copy number

product: inhibitor of DNA replication

essential: no

synonyms: yneE, yoxF

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]

[SW|Categories] containing this gene/protein

• [SW|DNA replication]

This gene is a member of the following [SW|regulons]

• [SW|Spo0A regulon]

Gene

Coordinates on the chromosome (coding sequence): 1,922,017 -> 1,922,463

Phenotypes of a mutant

• over-replication during sporulation [Pubmed|19682252]

The protein

Catalyzed reaction/ biological activity

• interacts with [protein|search|DnaA] and displaces it from the replication origin, resulting in inhibition of replication [Pubmed|19682252]

Structure

• [PDB|4TPS] (in complex with N-terminal domain of [protein|search|DnaA]) [Pubmed|25041308]

[SW|Localization]

• accumulates at the [SW|replisome] in sporulating cells (perhaps in [protein|search|DnaA]-dependent manner) [Pubmed|25041308]

[SW|Interactions]

• [protein|search|SirA]-[protein|search|DnaA] (N-terminal domain) [Pubmed|25041308,19682252,21239581]

Expression and Regulation

Operon

• ''[protein|search|sirA]-[protein|search|yneF]'' [Pubmed|9068642]

Regulation

• expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647,19329632]

Regulatory mechanism

• [SW|Spo0A]: transcription activation [Pubmed|14651647,19329632]

Biological materials

Mutant

• MGNA-B389 (yneE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1388 NBRP B. subtilis, Japan]

References

Reviews

• 20157337,22933559

Original publications

• 19682252,21239581,14651647,19329632,9068642,25041308,27489185