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sirA
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sirA

Sporulation protein, inhibits DNA replication initiation in cells committed to Sporulation, facilitates capture of oriC in the forespore
Locus
BSU_17900
Isoelectric point
7.35
Molecular weight
18.00 kDa
Protein length
148 aa Sequence Blast
Gene length
447 bp Sequence Blast
Function
control of chromosome copy number
Product
inhibitor of DNA replication
Essential
no
Synonyms
yneE,yoxF

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,922,017 1,922,463

Phenotypes of a mutant

  • over-replication during Sporulation PubMed
  • defective in oriC segregation during Sporulation PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • interacts with domain I of DnaA and displaces it from the replication origin, resulting in inhibition of replication PubMed
  • interacts with domain III of DnaA to facilitate ParA-dependent oriC capture in the forespore PubMed
  • Structure

  • 4TPS (in complex with N-terminal domain of DnaA) PubMed
  • Localization

  • accumulates at the replisome in sporulating cells (perhaps in DnaA-dependent manner) PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Regulatory mechanism

  • Spo0A: activation, PubMed, in Spo0A regulon
  • Regulation

  • expressed under conditions that trigger sporulation (Spo0A) PubMed
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    Biological materials

    Mutant

  • MGNA-B389 (yneE::erm), available at the NBRP B. subtilis, Japan
  • BKE17900 (sirA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTTCATCCCCTTTTC, downstream forward: _UP4_TAAAACCGAAGAAAAAGAGA
  • BKK17900 (sirA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTTCATCCCCTTTTC, downstream forward: _UP4_TAAAACCGAAGAAAAAGAGA
  • References

    Reviews

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    Original publications

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