sirA

[category|SW 4.2|Sporulation] protein, inhibits [category|SW 3.1.1|DNA replication] initiation in cells committed to [category|SW 4.2|Sporulation], facilitates capture of oriC in the forespore

locus: BSU_17900

pI: 7.35

mw: 18.00 kDa

protein length: 148 aa

gene length: 447 bp

function: control of chromosome copy number

product: inhibitor of [category|SW 3.1.1|DNA replication]

essential: no

synonyms: yneE,yoxF

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]

Gene

Coordinates: 1,922,017 1,922,463

Phenotypes of a mutant

• over-replication during [category|SW 4.2|Sporulation] [Pubmed|19682252]
• defective in oriC segregation during [category|SW 4.2|Sporulation] [pubmed|27489185]

The protein

Catalyzed reaction/ biological activity

• interacts with domain I of [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] and displaces it from the replication origin, resulting in inhibition of replication [Pubmed|19682252]
• interacts with domain III of [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] to facilitate [protein|5B3DB5A796ACA48390278E60478DFF13D0074A8D|ParA]-dependent oriC capture in the forespore [pubmed|27489185]

Structure

• [PDB|4TPS] (in complex with N-terminal domain of [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA]) [Pubmed|25041308]

[SW|Localization]

• accumulates at the [SW|replisome] in sporulating cells (perhaps in [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA]-dependent manner) [Pubmed|25041308]

Expression and Regulation

Operons

• genes: [gene|03595BF6DB8AE79604A720A5BD85C0DB781EE306|sirA]-[gene|9C151A07F22920F58D635B82545DA76C7087AA8E|yneF]
  description: [Pubmed|9068642]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [Pubmed|14651647,19329632], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

  regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647,19329632]

Biological materials

Mutant

• MGNA-B389 (yneE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1388 NBRP B. subtilis, Japan]
• BKE17900 ([gene|03595BF6DB8AE79604A720A5BD85C0DB781EE306|sirA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTCATCCCCTTTTC, downstream forward: _UP4_TAAAACCGAAGAAAAAGAGA
• BKK17900 ([gene|03595BF6DB8AE79604A720A5BD85C0DB781EE306|sirA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTCATCCCCTTTTC, downstream forward: _UP4_TAAAACCGAAGAAAAAGAGA

References

Reviews

• 20157337,22933559,28075389

Original publications

• 19682252,21239581,14651647,19329632,9068642,25041308,27489185,30285297