rpmE [2018-02-08T18:28:42.000Z]

ribosomal protein

Locus: BSU37070

Isoelectric point: 9.16

Molecular weight: 7.31 kDa

Protein length: 66 aa

Gene length: 198 bp

Function: translation

Product: ribosomal protein L31

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → Ribosomal proteins

Gene

Coordinates: 3,803,081 → 3,803,281

Phenotypes of a mutant

• delayed spore germination PubMed

The protein

Protein family

• Type A subfamily (according to Swiss-Prot)

Paralogous protein(s)

• RpmEB

Cofactors

• requires zinc for activity PubMed

Structure

• 3J9W (the ribosome) PubMed

Additional information

• Under conditions of zinc starvation, RpmE is replaced by its paralog RpmEB, that does not require zinc PubMed

Expression and Regulation

Operons

• Genes: rpmE
  Description: PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response
  • RpmE: autorepression, PubMed, in RpmE regulon

  Regulation

  • autorepression of rpmE expression upon binding of excess L31 to a RNA switch in the untranslated region of the rpmE mRNA PubMed
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Genes: rho-rpmE
  Description: PubMed

Biological materials

Mutant

• BKE37070 (ΔrpmE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGTATCCATCTCCTTCC, downstream forward: _UP4_TAATAATAGATTTCTCAACA
• BKK37070 (ΔrpmE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGTATCCATCTCCTTCC, downstream forward: _UP4_TAATAATAGATTTCTCAACA

References

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