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rpmE
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rpmE

ribosomal protein
Locus
BSU_37070
Isoelectric point
9.16
Molecular weight
7.31 kDa
Protein length
Gene length
201 bp Sequence Blast
Function
translation
Product
ribosomal protein L31
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,803,081 3,803,281

Phenotypes of a mutant

  • delayed spore germination PubMed
  • The protein

    Protein family

  • bacterial ribosomal protein bL31 family (with RpmEB, according to UniProt)
  • Paralogous protein(s)

    Cofactors

  • requires zinc for activity PubMed
  • Structure

    Additional information

  • Under conditions of zinc starvation, RpmE is replaced by its paralog RpmEB, that does not require zinc PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Regulatory mechanism

  • stringent response: negative regulation, in stringent response
  • RpmE: autorepression, PubMed, in RpmE regulon
  • Regulation

  • autorepression of rpmE expression upon binding of excess L31 to a RNA switch in the untranslated region of the rpmE mRNA PubMed
  • RelA dependent downregulation (Class I) during stringent response PubMed
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    Biological materials

    Mutant

  • BKE37070 (rpmE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGTATCCATCTCCTTCC, downstream forward: _UP4_TAATAATAGATTTCTCAACA
  • BKK37070 (rpmE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGTATCCATCTCCTTCC, downstream forward: _UP4_TAATAATAGATTTCTCAACA
  • References

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