yjiC [2019-11-15 15:56:27]

macrolide glycosyltransferase

locus: BSU_12220

pI: 4.65

mw: 43.83 kDa

protein length: 392 aa

gene length: 1179 bp

function: synthesis of bacillaene

product: macrolide glycosyltransferase

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.14|Resistance against toxins/ antibiotics/ based on similarity]

Gene

Coordinates: 1,292,557 1,293,735

The protein

Protein family

• UDP-glycosyltransferase family (with [protein|BD82663B1BF2763D2D2DE710C2896E4155419324|YdhE] and [protein|6D808BC20ADB6CA789288D50CA816D6BEE22CFC3|YojK], according to UniProt)

Structure

• [PDB|2IYA] (from Streptomyces antibioticus, 37% identity) [pubmed|17376874]

Expression and Regulation

Operons

• genes: [gene|D8E72C2523D5E6214C9CBAC2C1B27EA45378E162|yjiB]-[gene|02927D2CC9F44B99DB307DDFED2DD52DE2196120|yjiC]
  description: [pubmed|22383849]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [pubmed|26577401], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulation

  • induced by stress ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]) [pubmed|26577401]

Biological materials

Mutant

• MGNA-A345 (yjiC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/345 NBRP B. subtilis, Japan]
• BKE12220 ([gene|02927D2CC9F44B99DB307DDFED2DD52DE2196120|yjiC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAATCTCCAGTCTCCTT, downstream forward: _UP4_TAAAAACATAAAAACCGAAA
• BKK12220 ([gene|02927D2CC9F44B99DB307DDFED2DD52DE2196120|yjiC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAATCTCCAGTCTCCTT, downstream forward: _UP4_TAAAAACATAAAAACCGAAA

References

• 25354260,28315700,17376874,29338263