yobN [2019-08-05 08:50:22]

similar to L-amino acid oxidase

locus: BSU_19020

pI: 7.04

mw: 50.47 kDa

protein length: 446 aa

gene length: 1341 bp

function: unknown

product: unknown

essential: no

ec: 1.4.3.2

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.1|Spore coat proteins] → [category|SW 4.2.1.1.8|Not yet assigned]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 2,074,439 2,075,779

The protein

Catalyzed reaction/ biological activity

• An L-amino acid + H₂O + O₂ = a 2-oxo acid + NH₃ + H₂O₂ (according to Swiss-Prot)

Protein family

• flavin monoamine oxidase family (single member, according to UniProt)

[SW|Cofactors]

• FAD (according to UniProt)

Structure

• [PDB|3KVE] (from Vipera ammodytes 35% identity)

Expression and Regulation

Operons

• genes: [gene|02269068E6E4282A32BB5AE0FAE27F104202E5B5|yobN]-[gene|032110CBA492D54F6494984323D12D719EAEAAD9|yobO]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A311 (yobN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/311 NBRP B. subtilis, Japan]
• BKE19020 ([gene|02269068E6E4282A32BB5AE0FAE27F104202E5B5|yobN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTTGGAGGCACCTCT, downstream forward: _UP4_TAGATCATGCTCACATCTTG
• BKK19020 ([gene|02269068E6E4282A32BB5AE0FAE27F104202E5B5|yobN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTTGGAGGCACCTCT, downstream forward: _UP4_TAGATCATGCTCACATCTTG