SubtiBank SubtiBank
pyrE
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pyrE

orotate phosphoribosyltransferase
Locus
BSU_15560
Isoelectric point
5.63
Molecular weight
23.38 kDa
Protein length
216 aa Sequence Blast
Gene length
651 bp Sequence Blast
Function
pyrimidine biosynthesis
Product
orotate phosphoribosyltransferase
Essential
no
E.C.
2.4.2.10
Synonyms
pyrX
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of pyrimidine nucleotides

    • Gene

    Coordinates
    1,629,320 1,629,970

    The protein

    Catalyzed reaction/ biological activity

  • diphosphate + orotidine 5'-phosphate --> 5-phospho-α-D-ribose 1-diphosphate + orotate (according to UniProt)

    • Protein family

  • Purine/pyrimidine phosphoribosyltransferase family (according to UniProt)

    • Structure

  • 3DEZ (from Streptococcus mutans, 59% identity, 74% similarity)

    • Expression and Regulation

    Operons

    Genes
    pyrR-pyrP-pyrB-pyrC-pyrAA-pyrAB-pyrK-pyrD-pyrF-pyrE
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • PyrR: termination/ antitermination, via RNA switch, in PyrR regulon

    • Regulation

  • induced in the absence of uridine nucleotides (PyrR) PubMed
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    Biological materials

    Mutant

  • BKE15560 (pyrE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TAGATGTTTTGCGATGATTT, downstream forward: _UP4_TAAAAAATAAATTCAAATGA
  • BKK15560 (pyrE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TAGATGTTTTGCGATGATTT, downstream forward: _UP4_TAAAAAATAAATTCAAATGA

    • References

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