smc [2018-05-09 12:56:09]

part of the condensin complex, chromosomal origin condensation and segregation SMC protein

Locus: BSU15940

Isoelectric point: 5.27

Molecular weight: 135.29 kDa

Protein length: 1186 aa

Gene length: 3558 bp

Function: segregation of replication origins

Product: chromosome condensation and segregation SMC protein

Essential: yes

Synonyms: ylqA

Genomic Context

Loading

Categories containing this gene/protein

• Information processing → Genetics → DNA condensation/ segregation
• Groups of genes → Essential genes

Gene

Coordinates: 1,666,560 → 1,670,120

Phenotypes of a mutant

• essential PubMed
• terminal phenotype of a CRISPRi knockdown mutant is moderate lysis PubMed
• smc mutants are not viable on complex medim that allow rapid growth, but they are viable under conditions of slow growth PubMed
• smc lethality on complex medim can be suppressed by inactivation of tmcAL and tsaC (constitutive stringent response) PubMed

The protein

Protein family

• SMC family (according to Swiss-Prot)

Effectors of protein activity

• ATP-induced DNA-binding results in a more open conformation of the Smc dimer, and thus of the condensin complex PubMed

Structure

• 3ZGX (complex between the head domain of Smc (aa 1 - 219 and 983 - 1186) and the N-terminal winged-helix domain of ScpA) PubMed

Localization

• nucleoid (heterogeneous) PubMed
• the condensin (Smc)2-ScpA-ScpB complex is loaded by ParB to parS centromeric sites adjacent to the replication origin, travels then from the origin to the terminus at rates >50 kb per minute PubMed

Expression and Regulation

Operons

• Genes: rnc-smc-ftsY
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response

  Regulation

  • expressed during vegetative growth and during sporulation in the mother cell (SigK, GerE) PubMed

Biological materials

Mutant

• MGNA-B148 (smc::erm), available at the NBRP B. subtilis, Japan
• 1A822 ( smc::pMUTIN trpC2 Em), PubMed, available at the Bacillus Genetic Stock Center
• BEC15940 (smc::erm trpC2), a CRISPRi knockdown mutant, PubMed, is available at the Bacillus Genetic Stock Center

References

Reviews

Loading

Original publications

Loading