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smc
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smc

part of the [SW|condensin] complex, chromosomal origin condensation and segregation SMC protein
locus
BSU_15940
pI
5.27
mw
135.29 kDa
protein length
1186 aa Sequence Blast
gene length
3561 bp Sequence Blast
function
segregation of replication origins
product
chromosome condensation and segregation SMC protein
essential
yes
synonyms
ylqA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • Gene

    Coordinates
    1,666,560 1,670,120

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • terminal phenotype of a CRISPRi knockdown mutant is moderate lysis [Pubmed|27238023]
  • ''[gene|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc]'' mutants are not viable on complex medim that allow rapid growth, but they are viable under conditions of slow growth [Pubmed|24440399,24440393]
  • ''[gene|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc]'' lethality on complex medim can be suppressed by inactivation of ''[gene|0C76CAE27ACEBC0FF6B864B9F8C7D3C41C8D2A24|tmcAL]'' and ''[gene|EA155B3BEB9EAC97114058E23B6D8CDF8823A602|tsaC]'' (constitutive [SW|stringent response]) [Pubmed|26539825]

    • The protein

    Catalyzed reaction/ biological activity

  • ATPase activity, cycles of ATP hydrolysis are used by the [SW|condensin] complex to extrude DNA loops [pubmed|30100265]

    • Protein family

  • SMC family (with [protein|ED825D5B3290BA8F9C92477B1FCC433ADB3C8449|SbcC], according to UniProt)

    • [SW|Domains]

  • SMC hinge domain (aa 519-637) (according to UniProt)

    • Effectors of protein activity

  • ATP-induced DNA-binding results in a more open conformation of the [protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|Smc] dimer, and thus of the [SW|condensin] complex [Pubmed|25557547]

    • Structure

  • [PDB|3ZGX] (complex between the head domain of [protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|Smc] (aa 1 - 219 and 983 - 1186) and the N-terminal winged-helix domain of [protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|ScpA]) [Pubmed|23353789]

    • [SW|Localization]

  • nucleoid (heterogeneous) [Pubmed|16479537]
  • the [SW|condensin] ([protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|Smc])2-[protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|ScpA]-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|ScpB] complex is loaded by [protein|EF4BD49FCD49EE97908973581478951C8FA196C0|ParB] to ''parS'' centromeric sites adjacent to the replication origin, travels then from the origin to the terminus at rates >50 kb per minute [Pubmed|28154080,24440393,23475963]

    • Expression and Regulation

    Operons

    genes
    [gene|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|rnc]-[gene|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc]-[gene|D519765BF5261E48512CE86245BD7B733F188D4A|ftsY]
    description
    [Pubmed|11021934]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|11021934], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]

    • regulation

  • expressed during vegetative growth and during sporulation in the mother cell ([protein|search|SigK], [protein|search|GerE]) [Pubmed|11021934]
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    Biological materials

    Mutant

  • MGNA-B148 (smc::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1147 NBRP B. subtilis, Japan]
  • 1A822 ( ''smc''::pMUTIN ''trpC2'' Em), [Pubmed|12682299], available at [http://bgsc.org/getdetail.php?bgscid=1A822 the Bacillus Genetic Stock Center]
  • BEC15940 (''smc''::''erm trpC2''), a CRISPRi knockdown mutant, [Pubmed|27238023], is available at the [http://bgsc.org Bacillus Genetic Stock Center]

    • References

    Reviews

  • 19308706,22934648,22047950,22933559,24118085,25460803,26706151,29182912,27075410

    • Original publications

  • 27238023,9791128,12100548,17416649,19450516,12421306,11395470,19450517,19450516,15009890,16479537,21630458,23353789,25071173,22385855,25951515,26253537,24440399,24440393,23475963,25557547,25664627,26295962,26539825,26725510,26904953,28154080,28286005,28689660,30100265,30192981,31201090,31175837,32250245