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perR [2017-8-11 16:0:3]
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perR [2017-8-11 16:0:3]

transcriptional repressor of the peroxide regulon, sensor of the intracellular Fe/Mn ratio
locus
BSU08730
pI
5.89
mw
16.28 kDa
protein length
145 aa Sequence Blast
gene length
435 bp Sequence Blast
function
regulation of the response to peroxide
product
transcriptional repressor (Fur family)
essential
no
synonyms
ygaG
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

    • Gene

    Coordinates
    944,487 → 944,924

    Phenotypes of a mutant

  • resistant to hydrogen peroxide, accumulates a porphyrin-like compound, and grows very slowly (due to heme sequestration by [protein|B0A1AB6E920E6CBEE45115297599AF9A80972E38|KatA]) [Pubmed|22194458]

    • The protein

    Protein family

  • [SW|Fur family]

    • Paralogous protein(s)

  • [protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur], [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]

    • Modification

  • selective metal catalyzed oxidation of two histidine residues of the regulatory site results in induction (loss of DNA-binding activity) [Pubmed|19508285]

    • [SW|Cofactors]

  • contains an Fe(2+) at the regulatory site and Zn(2+) [Pubmed|22797754]

    • Effectors of protein activity

  • responds to the presence of hydrogen peroxide

    • Structure

  • [PDB|2RGV] [PDB|3F8N]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR]
    description
    [Pubmed|12029044]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12029044], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|00BCAAE16576DC5426A652580C69A570FC7A1C2C|PerR]: repression, [Pubmed|12029044], in [regulon|00BCAAE16576DC5426A652580C69A570FC7A1C2C|PerR regulon]

    • regulation

  • induced in the presence of hydrogen peroxide ([protein|search|PerR]) [Pubmed|12029044]
    • view in new tab

    genes
    [gene|3C139835154FA236066CF2FB82A3C32E3A72D65E|ygaF]-[gene|00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR]
    description
    [Pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • MGNA-A258 (ygaG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/258 NBRP B. subtilis, Japan]
  • HB0509 (spc), available in [SW|John Helmann]'s and [SW|Jörg Stülke]'s labs, also GP868 (''fur::mls'', ''perR::spc'').
  • 1A903 ( ''perR''::''kan''), [Pubmed|12029044], available at the [http://bgsc.org/getdetail.php?bgscid=1A903 Bacillus Genetic Stock Center]
  • BKE08730 (''perR''::''erm trpC2'') is available at the [http://bgsc.org/ Bacillus Genetic Stock Center] [pubmed|28189581]

    • Labs working on this gene/protein

  • [SW|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]

    • References

    Reviews

  • 19575568,19508286,20094649,20977351,22797754,25160631,26070785,26677871

    • Original Publications

  • 8932315,18487332,14563870,16766519,17158660,12486061,11532148,12180919,16166527,15231799,12029044,10913706,21398634,9701813,16541078,12029044,11532148,12029044,19508285,22194458,23645680,23057863,23934352,25486128,26261021,27920297,27935957,28104400, 11918677