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perR [Tue Dec 13 2016 11:16:31 GMT+0100 (CET)]
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perR [Tue Dec 13 2016 11:16:31 GMT+0100 (CET)]

transcriptional repressor of the peroxide regulon, sensor of the intracellular Fe/Mn ratio
locus
BSU08730
pI
5.00
mw
16.00 kDa
function
regulation of the response to peroxide
product
transcriptional repressor (Fur family)
essential
no
synonyms
ygaG
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

    • [SW|Categories] containing this gene/protein

  • [SW|transcription factors and their control], [SW|resistance against oxidative and electrophile stress]

    • This gene is a member of the following [SW|regulons]

  • [SW|PerR regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    944,487 -> 944,924

    Phenotypes of a mutant

  • resistant to hydrogen peroxide, accumulates a porphyrin-like compound, and grows very slowly (due to heme sequestration by [protein|search|KatA]) [Pubmed|22194458]

    • The protein

    Protein family

  • [SW|Fur family]

    • Paralogous protein(s)

  • [protein|search|Zur], [protein|search|Fur]

    • Modification

  • selective metal catalyzed oxidation of two histidine residues of the regulatory site results in induction (loss of DNA-binding activity) [Pubmed|19508285]

    • [SW|Cofactors]

  • contains an Fe(2) at the regulatory site and Zn(2) [Pubmed|22797754]

    • Effectors of protein activity

  • responds to the presence of hydrogen peroxide

    • Structure

  • [PDB|2RGV] [PDB|3F8N]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operon

  • ''perR'' [Pubmed|12029044]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|12029044]

    • Regulation

  • negative autoregulation [Pubmed|12029044]
  • induced in the presence of hydrogen peroxide ([protein|search|PerR]) [Pubmed|12029044]

    • Regulatory mechanism

  • [protein|search|PerR]: transcription repression [Pubmed|12029044]

    • Biological materials

    Mutant

  • MGNA-A258 (ygaG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/258 NBRP B. subtilis, Japan]
  • HB0509 (spc), available in [SW|John Helmann]'s and [SW|Jörg Stülke]'s labs, also GP868 (''fur::mls'', ''perR::spc'').
  • 1A903 ( ''perR''::''kan''), [Pubmed|12029044], available at the [http://bgsc.org/getdetail.php?bgscid=1A903 Bacillus Genetic Stock Center]
  • BKE08730 (''perR''::''erm trpC2'') is available at the [http://bgsc.org/ Bacillus Genetic Stock Center]

    • Labs working on this gene/protein

  • [SW|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]

    • References

    Reviews

  • 19575568,19508286,20094649,20977351,22797754,25160631,26070785,26677871

    • Original Publications

  • 8932315,18487332,14563870,16766519,17158660,12486061,11532148,12180919,16166527,15231799,12029044,10913706,21398634,9701813,16541078,12029044,11532148,12029044,19508285,22194458,23645680,23057863,23934352,25486128,26261021,27920297