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niaP [2019-06-18 11:00:39]
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niaP [2019-06-18 11:00:39]

nicotinate transporter
locus
BSU02950
pI
9.50
mw
43.55 kDa
protein length
400 aa Sequence Blast
gene length
1203 bp Sequence Blast
function
uptake of niacin
product
nicotinate transporter
essential
no
synonyms
yceI
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.7|Transporter for cofactors]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.11|Biosynthesis of NAD(P)]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    317,725 318,927

    Phenotypes of a mutant

  • more sensitive to nisin [Pubmed|23980836]

    • The protein

    Protein family

  • [SW|major facilitator superfamily] [Pubmed|18276644]

    • [SW|Domains]

  • 10 membrane-spanning domains [Pubmed|18276644]

    • Structure

  • [PDB|4J05] (from ''P. indica'', 24% identity) [Pubmed|23542591]

    • [SW|Localization]

  • cell membrane [Pubmed|18763711]

    • Expression and Regulation

    Operons

    genes
    [gene|0037A6808E75C2A2E207A7005EFDA71FCE90BAD8|niaP]
    description
    [Pubmed|22383849]

    regulatory mechanism

  • [protein|510DE075EA964D22C42DEB171509F6CDA600A402|NadR]: repression, [Pubmed|18276644], in [regulon|510DE075EA964D22C42DEB171509F6CDA600A402|NadR regulon]

    • regulation

  • repressed in the presence of nicotinic acid ([protein|search|NadR]) [Pubmed|18276644]
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    Biological materials

    Mutant

  • MGNA-C047 (yceI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2045 NBRP B. subtilis, Japan]
  • BKE02950 ([gene|0037A6808E75C2A2E207A7005EFDA71FCE90BAD8|niaP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCCAACACCTCTGATA, downstream forward: _UP4_TAGGAAAAGCACCTCTTAAA
  • BKK02950 ([gene|0037A6808E75C2A2E207A7005EFDA71FCE90BAD8|niaP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCCAACACCTCTGATA, downstream forward: _UP4_TAGGAAAAGCACCTCTTAAA

    • References

    Reviews

  • 21953179

    • Original publications

  • 18763711,18276644,23980836,22383849,23542591