tkt [Sun Nov 27 2016 17:42:49 GMT+0100 (CET)]

transketolase

locus: BSU17890

pI: 4.00

mw: 72.00 kDa

function: pentose phosphate pathway

product: transketolase

essential: no

ec: 2.2.1.1

synonyms: tktA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.3|Pentose phosphate pathway]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

[SW|Categories] containing this gene/protein

• [SW|carbon core metabolism], [SW|phosphoproteins], [SW|most abundant proteins]

This gene is a member of the following [SW|regulons]

• [SW|Spo0A regulon]

Gene

Coordinates on the chromosome (coding sequence): 1,919,861 -> 1,921,864

The protein

Catalyzed reaction/ biological activity

• Sedoheptulose 7-phosphate D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate D-xylulose 5-phosphate (according to Swiss-Prot)

Protein family

• transketolase family (according to Swiss-Prot)

Modification

• phosphorylated on ser/ thr/ tyr [Pubmed|16493705]

[SW|Cofactors]

• thiamine pyrophosphate

Structure

• [PDB|search|3K95], complex with thiamine diphosphate, from ''B. anthracis

[SW|Interactions]

• [protein|search|Tkt]-[protein|search|Tpi] [Pubmed|19193632]

Expression and Regulation

Operon

• ''tkt'' [Pubmed|9068642]

Regulation

• repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]

Regulatory mechanism

• [SW|Spo0A]: transcription repression [Pubmed|14651647]

Additional information

• belongs to the 100 [[most abundant proteins]] [PubMed|15378759]

Biological materials

Mutant

• BS4530 (aphA3), available in [SW|Stülke] lab

Expression vector

• pGP94 (N-terminal Strep-tag, for [SW|SPINE], expression in B. subtilis, in [SW|pGP380]), available in [SW|Stülke] lab
• for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP820, available in [SW|Stülke] lab

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Stülke] lab

FLAG-tag construct

• GP1406 (spc, based on [SW|pGP1331]), available in the [SW|Stülke] lab

Antibody

• **

References

Reviews

• 9924800

Original publications

• 14651647,16493705,19603213,9068642,16143417,15375635,19193632,23568212,23965678,15378759