tkt

transketolase

Locus: BSU_17890

Isoelectric point: 4.80

Molecular weight: 72.16 kDa

Protein length: 667 aa

Gene length: 2004 bp

Function: pentose phosphate pathway

Product: transketolase

Essential: no

E.C.: 2.2.1.1

Synonyms: tktA

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Carbon core metabolism → Pentose phosphate pathway
• Groups of genes → Phosphoproteins → Phosphorylation on either a Ser, Thr or Tyr residue

Gene

Coordinates: 1,919,861 1,921,864

The protein

Catalyzed reaction/ biological activity

• D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate --> aldehydo-D-ribose 5-phosphate + D-xylulose 5-phosphate (according to UniProt)

Protein family

• transketolase family (with Dxs, according to UniProt)

Modification

• phosphorylated on ser/ thr/ tyr PubMed

Cofactors

• thiamine pyrophosphate

Structure

• 3HYL, from B. anthracis

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: tkt
  Description: PubMed

  Regulatory mechanism

  • Spo0A: repression, PubMed, in Spo0A regulon

  Regulation

  • repressed under conditions that trigger sporulation (Spo0A) PubMed

Biological materials

Mutant

• BS4530 (tkt::aphA3), available in Jörg Stülke's lab
• BKE17890 (tkt::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAAAATCCCCTTCCTTA, downstream forward: _UP4_TAAGCTTTTGAAAGAGGATG
• BKK17890 (tkt::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAAAATCCCCTTCCTTA, downstream forward: _UP4_TAAGCTTTTGAAAGAGGATG

Expression vectors

• pGP94 (N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380), available in Jörg Stülke's lab
• for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP820, available in Jörg Stülke's lab, PubMed

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

FLAG-tag construct

• GP1406 (spc, based on pGP1331), available in Jörg Stülke's lab

References

Reviews

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Original publications

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