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sigM
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sigM

RNA polymerase ECF-type sigma factor SigM, required for adaptation to inhibitors of peptidoglycan synthesis
Locus
BSU_09520
Isoelectric point
6.77
Molecular weight
19.26 kDa
Protein length
163 aa Sequence Blast
Gene length
492 bp Sequence Blast
Function
adaptation to inhibitors of peptidoglycan synthesis
Product
RNA polymerase ECF-type sigma factor SigM
Essential
no
Synonyms
yhdM
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → RNA synthesis and degradation → Transcription → Sigma factors
  • Information processing → Regulation of gene expression → Sigma factors and their control → Sigma factors
  • Lifestyles → Coping with stress → Resistance against toxins/ antibiotics
  • Lifestyles → Coping with stress → Cell envelope stress proteins (controlled by SigM, V, W, X, Y)

    • Gene

    Coordinates
    1,029,577 1,030,068

    Phenotypes of a mutant

  • SigM is essential for growth and survival in nutrient broth (NB) containing 1.4 M NaCl PubMed
  • sigM mutants form aberrantly shaped cells, which swell and lyse spontaneously during growth in NB medium containing increased levels (0.35-0.7 M) of a wide range of different salts PubMed
  • increased sensitivity towards beta-lactam antibiotics PubMed
  • a sigM murJ double mutant is not viable (due to the lack of both lipid II flippases) PubMed

    • The protein

    Protein family

  • Sigma-70 factor family (according to UniProt)
  • ECF subfamily (according to UniProt)

    • Effectors of protein activity

  • expression of CsbB in the absence of YfhO causes constitutive activation of SigM PubMed
  • shortage of available bactoprenol causes SigM activation PubMed

    • Structure

  • 5WUQ (SigW in complex with the cytoplasmic domain of RsiW, 32% identity) PubMed

    • Additional information

  • Expression of the SigM regulon in increased in ugtP mutants PubMed
  • information on binding sites can be found in the PRODORIC2 database

    • Expression and Regulation

    Operons

    Genes
    sigM-yhdL-yhdK
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigM: sigma factor, PubMed, in SigM regulon

    • Regulation

  • induced by glucose PubMed

    • Additional information

  • expression of CsbB in the absence of YfhO causes constitutive activation of SigM PubMed
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    Biological materials

    Mutant

  • 1A906 (sigM::kan), PubMed, available at BGSC
  • BP97 (Δ(sigM-yhdL-yhdK)::aphA3), available in Fabian Commichau's lab
  • BP129 (ΔsigM::aphA3), available in Fabian Commichau's lab
  • BKE09520 (sigM::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTGCCTTTTCTCCCCTCT, downstream forward: _UP4_AAAGCACTTTATAATAGAGG
  • BKK09520 (sigM::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTGCCTTTTCTCCCCTCT, downstream forward: _UP4_AAAGCACTTTATAATAGAGG

    • Labs working on this gene/protein

  • John Helmann, Cornell University, USA Homepage

    • References

    Reviews

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    The SigM regulon

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    Other original publications

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