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sigE
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sigE

RNA polymerase sporulation mother cell-specific (early) sigma factor SigE
Locus
BSU_15320
Isoelectric point
9.21
Molecular weight
27.55 kDa
Protein length
239 aa Sequence Blast
Gene length
720 bp Sequence Blast
Function
transcription of sporulation genes (early mother cell)
Product
RNA polymerase sporulation mother cell-specific (early) sigma factor SigE
Essential
no
Synonyms
spoIIGB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → RNA synthesis and degradation → Transcription → Sigma factors
  • Information processing → Regulation of gene expression → Sigma factors and their control → Sigma factors
  • Groups of genes → Efp-dependent proteins

    • Gene

    Coordinates
    1,604,771 1,605,490

    The protein

    Catalyzed reaction/ biological activity

  • RNA polymerase sigma factor, active in the mother cell

    • Protein family

  • Sigma-70 factor family (according to UniProt)

    • Structure

  • 1L0O (SigF in complex with SpoIIAB, Geobacillus stearothermophilus, 33% identity) PubMed

    • Expression and Regulation

    Operons

    Genes
    spoIIGA-sigE-sigG
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigG: sigma factor, PubMed, in SigG regulon
  • SigF: sigma factor, in SigF regulon

    • Regulatory mechanism

  • SinR: repression, in SinR regulon
  • Spo0A: activation, PubMed, in Spo0A regulon
  • AbrB: repression, PubMed, in AbrB regulon

    • Regulation

  • spoIIGA: expressed under conditions that trigger sporulation (Spo0A) PubMed

    • Additional information

  • the mRNA half-life is about 2.6 min PubMed
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    Additional information

  • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

    • Biological materials

    Mutant

  • BKE15320 (sigE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTCCTCTCCCTTCTAA, downstream forward: _UP4_TAAAAAATTTTATGGTTAGA
  • BKK15320 (sigE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTCCTCTCCCTTCTAA, downstream forward: _UP4_TAAAAAATTTTATGGTTAGA

    • Labs working on this gene/protein

  • Bill Haldenwang, San Antonio, USA
  • Charles Moran, Emory University, NC, USA homepage

    • References

    Reviews

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    The SigE regulon

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    Original Publications

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