SubtiBank SubtiBank
licT
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

licT

transcriptional antiterminator of the bglP-bglH-yxiE operon and the bglS gene
Locus
BSU_39080
Isoelectric point
5.94
Molecular weight
32.22 kDa
Protein length
277 aa Sequence Blast
Gene length
834 bp Sequence Blast
Function
control of beta-glucan and beta-glucoside utilization
Product
transcriptional antiterminator (BglG family)
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
Loading

Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of beta-glucosides
  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of other polymeric carbohydrates
  • Information processing → Regulation of gene expression → Transcription factors and their control → PRD-type regulators
  • Information processing → Regulation of gene expression → RNA binding regulators
  • Groups of genes → Phosphoproteins → Phosphorylation on a His residue

    • Gene

    Coordinates
    4,012,866 → 4,013,699

    Phenotypes of a mutant

  • no expression of the bglP-bglH operon

    • The protein

    Catalyzed reaction/ biological activity

  • binding to the mRNAs of bglS and the bglP-bglH operon, causes transcription antitermination (in presence of salicin and absence of glucose)

    • Protein family

  • PRD-containing transcription factors

    • Paralogous protein(s)

  • SacT, GlcT, SacY

    • Domains

  • N-terminal RNA binding domain PubMed
  • 2 x PRD (PTS regulation domains) PubMed
  • 2 PRD domains (aa 65-170, aa 171-277) (according to UniProt)

    • Modification

  • phosphorylation at His-100 in PRD-1 by phosphorylated BglP, inhibits LicT antitermination activity
  • phosphorylation at His-207 and/or His-269 in PRD-2 by His-P-HPr, stimulates LicT antitermination activity

    • Structure

  • 1L1C (complex with RAT)
  • 1TLV (PRDs)

    • Localization

  • cytoplasm, even distribution in the absence of the inducer salicin, subpolar localization in the presence of salicin PubMed

    • Additional information

  • information on binding sites can be found in the PRODORIC2 database

    • Expression and Regulation

    Operons

    Genes
    licT-bglS
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulation

  • expressed in the stationary phase (temporal activation) PubMed
    • view in new tab

    Genes
    yxiP-licT-bglS
    Description
    PubMed
    view in new tab

    Biological materials

    Mutant

  • GP427 (ΔlicT-bglS::erm), available in Jörg Stülke's lab
  • BKE39080 (ΔlicT::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGCATGTCCCTCCAAAT, downstream forward: _UP4_TAATGAGAGCGCTGACATTT
  • BKK39080 (ΔlicT::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGCATGTCCCTCCAAAT, downstream forward: _UP4_TAATGAGAGCGCTGACATTT

    • Expression vectors

  • for expression, purification of both PRDs in E. coli with N-terminal His-tag, in pWH844: pGP165, available in Jörg Stülke's lab
  • for expression, purification of RNA-binding domain in E. coli with N-terminal His-tag, in pWH844: pGP315, available in Jörg Stülke's lab
  • for expression, purification of RNA-binding domain in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP572, available in Jörg Stülke's lab

    • GFP fusion

  • GP1225, licT-gfp, spc, based on pGP1870), available in Jörg Stülke's lab PubMed
  • GP1229, licT-yfp, spc), available in Jörg Stülke's lab PubMed

    • FLAG-tag construct

  • GP1221 (spc, based on pGP1331), available in Jörg Stülke's lab

    • Labs working on this gene/protein

  • Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
  • Josef Deutscher, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

    • References

    Reviews

    Loading

    Original publications

    Loading