SubtiBank SubtiBank
holB
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holB

DNA polymerase III (delta subunit), part of the clamp-loader complex and the replisome
Locus
BSU_00310
Isoelectric point
6.33
Molecular weight
37.47 kDa
Protein length
329 aa Sequence Blast
Gene length
990 bp Sequence Blast
Function
DNA replication
Product
DNA polymerase III (delta subunit)
Essential
yes
E.C.
2.7.7.7
Synonyms
yaaS
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Genetics → DNA replication
  • Groups of genes → Essential genes

    • Gene

    Coordinates
    40,665 → 41,654

    Phenotypes of a mutant

  • essential PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • deoxyribonucleoside 5'-triphosphate + DNA(n) --> diphosphate + DNA(n+1) (according to UniProt)
  • required for bacteriophage SPP1 replication PubMed

    • Structure

  • 1NJG (from E. coli, 36% identity) PubMed

    • Localization

  • forms foci PubMed
  • cytoplasm (homogeneous) PubMed

    • Expression and Regulation

    Operons

    Genes
    xpaC-yaaN-yaaO-tmk-darA-yaaR-holB-yaaT-yabA-trmN6-yazA-yabC
    Description
    PubMed
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    Genes
    darA-yaaR-holB-yaaT-yabA-trmN6-yazA-yabC
    Description
    PubMed

    Regulation

  • expressed during growth and the transition phase, expression is erduced in stationary phase PubMed
    • view in new tab

    Genes
    tmk-darA-yaaR-holB-yaaT-yabA-trmN6-yazA-yabC
    Description
    PubMed
    view in new tab

    Biological materials

    Expression vectors

  • pGP1692: expression of Strep-holB-Strep in E. coli, in pGP172, available in Jörg Stülke's lab
  • pGP1698: expression of holB-Strep in E. coli, in pGP574, available in Jörg Stülke's lab
  • pGP1699: expression of holB in E. coli, in pGP574 (no tag due to a stop codon at the end of holB), available in Jörg Stülke's lab
  • pGP2672: expression of Strep-holB by pGP380 in B. subtilis, suitable for SPINE, available in Jörg Stülke's lab
  • pGP3028: expression of holB-Strep by pGP382 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

    • References

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