SubtiBank SubtiBank
holB
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holB

DNA polymerase III (delta subunit), part of the clamp-loader complex and the replisome
Locus
BSU_00310
Isoelectric point
6.33
Molecular weight
37.47 kDa
Protein length
329 aa Sequence Blast
Gene length
990 bp Sequence Blast
Product
DNA polymerase III (delta subunit)
Essential
yes
E.C.
2.7.7.7
Synonyms
yaaS

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
40,665 → 41,654

Phenotypes of a mutant

  • essential PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • deoxyribonucleoside 5'-triphosphate + DNA(n) --> diphosphate + DNA(n+1) (according to UniProt)
  • required for bacteriophage SPP1 replication PubMed
  • Structure

  • 1NJG (from E. coli, 36% identity) PubMed
  • Localization

  • forms foci PubMed
  • cytoplasm (homogeneous) PubMed
  • Expression and Regulation

    Operons

    Description

    Regulation

  • expressed during growth and the transition phase, expression is erduced in stationary phase PubMed
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    Biological materials

    Expression vectors

  • pGP1692: expression of Strep-holB-Strep in E. coli, in pGP172, available in Jörg Stülke's lab
  • pGP1698: expression of holB-Strep in E. coli, in pGP574, available in Jörg Stülke's lab
  • pGP1699: expression of holB in E. coli, in pGP574 (no tag due to a stop codon at the end of holB), available in Jörg Stülke's lab
  • pGP2672: expression of Strep-holB by pGP380 in B. subtilis, suitable for SPINE, available in Jörg Stülke's lab
  • pGP3028: expression of holB-Strep by pGP382 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
  • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • References

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