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holB
DNA polymerase III (delta subunit), part of the clamp-loader complex and the
replisomeMolecular weight
37.47 kDa
Product
DNA polymerase III (delta subunit)
Genomic Context
Categories containing this gene/protein
Gene
Coordinates
40,665 → 41,654
Phenotypes of a mutant
The protein
Catalyzed reaction/ biological activity
deoxyribonucleoside 5'-triphosphate + DNA(n) --> diphosphate + DNA(n+1) (according to UniProt)required for bacteriophage SPP1 replication PubMed Structure
Localization
Expression and Regulation
Operons
Regulation
expressed during growth and the transition phase, expression is erduced in stationary phase PubMed view in new tabBiological materials
Expression vectors
pGP1692: expression of Strep-holB-Strep in E. coli, in pGP172, available in Jörg Stülke's labpGP1698: expression of holB-Strep in E. coli, in pGP574, available in Jörg Stülke's labpGP1699: expression of holB in E. coli, in pGP574 (no tag due to a stop codon at the end of holB), available in Jörg Stülke's labpGP2672: expression of Strep-holB by pGP380 in B. subtilis, suitable for SPINE, available in Jörg Stülke's labpGP3028: expression of holB-Strep by pGP382 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab References
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