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gltB
small subunit of glutamate synthase
Molecular weight
54.78 kDa
Function
glutamate biosynthesis
Product
glutamate synthase (small subunit)
Genomic Context
Categories containing this gene/protein
Gene
Coordinates
2,008,572 2,010,053
Phenotypes of a mutant
auxotrophic for glutamateThe protein
Catalyzed reaction/ biological activity
2 L-glutamate + NADP+ --> 2-oxoglutarate + H+ + L-glutamine + NADPH (according to UniProt)Protein family
glutamate synthase family (with GltA and YerD, according to UniProt) Domains
nucleotide binding domain (NADP) (299313)Structure
Localization
cytoplasm (according to Swiss-Prot)Expression and Regulation
Operons
Other regulations
FsrA: translation inhibition, Biological materials
Mutant
BP123 (ΔgltA-gltB::ermC) , available in Fabian Commichau's labGP807 (ΔgltA-gltB::tet) , available in Jörg Stülke's labGP517 (ΔgltB::ermC), available in Jörg Stülke's labBKE18440 (gltB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTCCTCTCCCCTTCCT, downstream forward: _UP4_TAAATAAAGGGGATTATCATBKK18440 (gltB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTCCTCTCCCCTTCCT, downstream forward: _UP4_TAAATAAAGGGGATTATCAT Expression vectors
pGP1119 (in pGP380, for SPINE, expression in B. subtilis), available in Jörg Stülke's lab PubMedpGP3031: IPTG inducible expression, purification in E. coli with C-terminal His-tag, in pGP574 (Strep-tag omitted), available in Jörg Stülke's labpGP3032: IPTG inducible expression, purification in E. coli with C-terminal Strep-tag, in pGP574, available in Jörg Stülke's lab LacZ fusion
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab Labs working on this gene/protein
References
Reviews
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Original publications
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