SubtiBank SubtiBank
gltB
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gltB

small subunit of glutamate synthase
Locus
BSU_18440
Isoelectric point
7.70
Molecular weight
54.78 kDa
Protein length
493 aa Sequence Blast
Gene length
1482 bp Sequence Blast
Function
glutamate biosynthesis
Product
glutamate synthase (small subunit)
Essential
no
E.C.
1.4.1.13
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation

    • Gene

    Coordinates
    2,008,572 2,010,053

    Phenotypes of a mutant

  • auxotrophic for glutamate

    • The protein

    Catalyzed reaction/ biological activity

  • 2 L-glutamate + NADP+ --> 2-oxoglutarate + H+ + L-glutamine + NADPH (according to UniProt)

    • Protein family

  • glutamate synthase family (with GltA and YerD, according to UniProt)

    • Domains

  • nucleotide binding domain (NADP) (299313)

    • Structure

  • 2VDC (the GltA-GltB complex of Azospirillum brasiliense) PubMed

    • Localization

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    Genes
    gltA-gltB
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • TnrA: repression, PubMed, in TnrA regulon
  • GltC: activation, PubMed, in GltC regulon
  • Fur: indirect effect, in Fur regulon

    • Regulation

  • expressed in the presence of ammonium PubMed
    • view in new tab

    Other regulations

  • FsrA: translation inhibition,

    • Biological materials

    Mutant

  • BP123 (ΔgltA-gltB::ermC) , available in Fabian Commichau's lab
  • GP807 (ΔgltA-gltB::tet) , available in Jörg Stülke's lab
  • GP517 (ΔgltB::ermC), available in Jörg Stülke's lab
  • BKE18440 (gltB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTCCTCTCCCCTTCCT, downstream forward: _UP4_TAAATAAAGGGGATTATCAT
  • BKK18440 (gltB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTCCTCTCCCCTTCCT, downstream forward: _UP4_TAAATAAAGGGGATTATCAT

    • Expression vectors

  • pGP1119 (in pGP380, for SPINE, expression in B. subtilis), available in Jörg Stülke's lab PubMed
  • pGP3031: IPTG inducible expression, purification in E. coli with C-terminal His-tag, in pGP574 (Strep-tag omitted), available in Jörg Stülke's lab
  • pGP3032: IPTG inducible expression, purification in E. coli with C-terminal Strep-tag, in pGP574, available in Jörg Stülke's lab

    • LacZ fusion

  • see ''gltA

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

    • Labs working on this gene/protein

  • Linc Sonenshein, Tufts University, Boston, MA, USA Homepage
  • Fabian Commichau, Göttingen, Germany homepage

    • References

    Reviews

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    Original publications

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