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gltA
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gltA

large subunit of glutamate synthase
Locus
BSU_18450
Isoelectric point
5.47
Molecular weight
168.61 kDa
Protein length
1520 aa Sequence Blast
Gene length
4563 bp Sequence Blast
Function
glutamate biosynthesis
Product
glutamate synthase (large subunit)
Essential
no
E.C.
1.4.1.13
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,010,070 2,014,632

Phenotypes of a mutant

  • auxotrophic for glutamate
  • The protein

    Catalyzed reaction/ biological activity

  • 2 L-glutamate + NADP+ --> 2-oxoglutarate + H+ + L-glutamine + NADPH (according to UniProt)
  • Protein family

  • glutamate synthase family (with YerD and GltB, according to UniProt)
  • Paralogous protein(s)

    Domains

  • Glutamine amidotransferase type-2 domain (aa 22-415) (according to UniProt)
  • Nucleotide binding domain (1060-1112)
  • Modification

  • phosphorylated on Arg-904 AND/OR Arg-914 PubMed
  • Cofactors

  • Fe-S cluster PubMed
  • FAD (according to UniProt)
  • Structure

  • 2VDC (the GltA-GltB complex of Azospirillum brasiliense) PubMed
  • Localization

  • membrane associated PubMed, cytoplasm (according to UniProt)
  • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed
  • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • TnrA: repression, PubMed, in TnrA regulon
  • GltC: activation, PubMed, in GltC regulon
  • Fur: indirect effect, in Fur regulon
  • Regulation

  • expressed in the presence of ammonium PubMed
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    Other regulations

  • FsrA: translation inhibition, PubMed
  • Biological materials

    Mutant

  • BP123 (ΔgltA-gltB::ermC) , available in Fabian Commichau's lab
  • GP807 (ΔgltA-gltB::tet) , available in Jörg Stülke's lab
  • GP222 (gltA under the control of p-xyl), available in Jörg Stülke's lab
  • 1A808 ( gltA::cat), PubMed, available at BGSC
  • 1A809 ( gltA::kan), PubMed, available at BGSC
  • BKE18450 (gltA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAATTCCTCTCCCCCGAT, downstream forward: _UP4_GTACAGTAAGGAAGGGGAGA
  • BKK18450 (gltA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAATTCCTCTCCCCCGAT, downstream forward: _UP4_GTACAGTAAGGAAGGGGAGA
  • LacZ fusion

  • pGP526 (in pAC7), available in Jörg Stülke's lab PubMed
  • pGP919 (in pAC5), available in Jörg Stülke's lab PubMed
  • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Labs working on this gene/protein

  • Linc Sonenshein, Tufts University, Boston, MA, USA Homepage
  • Jörg Stülke, University of Göttingen, Germany Homepage
  • Fabian Commichau Brandenburg Technical University Cottbus-Senftenberg, Germany Homepage
  • References

    Reviews

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    Original publications

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