SubtiBank SubtiBank
comK
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

comK

competence transcription factor (CTF)
Locus
BSU_10420
Isoelectric point
7.67
Molecular weight
22.28 kDa
Protein length
192 aa Sequence Blast
Gene length
579 bp Sequence Blast
Function
regulation of genetic competence and DNA uptake
Product
competence transcription factor (CTF)
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
Loading

Categories containing this gene/protein

  • Information processing → Genetics → Genetic competence
  • Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other
  • Lifestyles → Exponential and early post-exponential lifestyles → Genetic competence
  • Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

    • Gene

    Coordinates
    1,117,109 1,117,687

    Phenotypes of a mutant

  • no expression of competence genes, loss of genetic competence PubMed

    • The protein

    Modification

  • phosphorylated on Arg-65, Arg-157, Arg-161, Arg-165, Arg-186, and Arg-191 PubMed

    • Additional information

  • degraded by ClpC-ClpP (with MecA as adaptor protein) PubMed
  • information on binding sites can be found in the PRODORIC2 database

    • Expression and Regulation

    Operons

    Genes
    comK
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • Rok: repression, PubMed, in Rok regulon
  • CodY: repression, PubMed, in CodY regulon
  • AbrB: repression, PubMed, in AbrB regulon
  • ComK: activation, (autoregulation) PubMed, in ComK regulon
  • DegU: activation, in DegU regulon
  • Spo0A: activation, Spo0A-P, acts as antagonist of Rok PubMed, in Spo0A regulon

    • Regulation

  • comK has a bistable expression pattern PubMed
  • expression is reduced in a cwlO mutant PubMed
    • view in new tab

    Additional information

  • full expression of comK requires fully assembled and rotating flagella PubMed
  • DegU-P represses comK expression PubMed

    • Biological materials

    Mutant

  • 1A871 (no resistance), [Pubmed| ], available at BGSC
  • BKE10420 (comK::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTATGGCCTCCATCCT, downstream forward: _UP4_TAGAAAAATAGGAAGGAGCT
  • BKK10420 (comK::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTATGGCCTCCATCCT, downstream forward: _UP4_TAGAAAAATAGGAAGGAGCT

    • Expression vectors

  • for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

    • Labs working on this gene/protein

  • Oscar Kuipers, University of Groningen, The Netherlands
  • Homepage

    • References

    Reviews

    Loading

    The ComK regulon

    Loading

    Original Publications

    Loading