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bceA
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bceA

primary bacitracin resistance determinant, ABC transporter (ATP-binding protein), protects cell wall biosynthetic targets from inhibition by antimicrobial peptides
Locus
BSU_30380
Isoelectric point
9.29
Molecular weight
28.08 kDa
Protein length
253 aa Sequence Blast
Gene length
762 bp Sequence Blast
Function
protection of cell wall biosynthetic targets from inhibition by antimicrobial peptides
Product
ABC transporter (ATP-binding protein) for target protection of cell wall synthesis
Essential
no
Synonyms
ytsC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → ABC transporters → Exporters → Export of peptides
  • Lifestyles → Coping with stress → Resistance against toxins/ antibiotics
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    3,111,327 3,112,088

    Phenotypes of a mutant

  • 85-fold increased sensitivity to bacitracin PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • releaves intermediates of the lipid II cycle from the inhibitory interaction with antimicrobial peptides such as bacitracin PubMed

    • Protein family

  • ABC transporter family (according to UniProt)

    • Paralogous protein(s)

  • YknY, YtrE, YxdL, PsdA, YvrO

    • Domains

  • ABC transporter domain (aa 4-243) (according to UniProt)

    • Effectors of protein activity

  • the activity of the BceA-BceB ABC transporter is inhibited by heptaprenyl pyrophosphate which accumulates in a ytpB mutant PubMed

    • Structure

  • 1L2T (MJ0796 from Methanocaldococcus jannaschii , 38% identity) PubMed

    • Localization

  • membrane associated (via BceB) PubMed

    • Expression and Regulation

    Operons

    Genes
    bceA-bceB
    Description
    PubMed

    Regulatory mechanism

  • BceR: activation, PubMed, in BceR regulon

    • Regulation

  • induced in the presence of bacitracin, plectasin, mersacidin and actagardine, already at very low levels of bacitracin (BceR) PubMed
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    Biological materials

    Mutant

  • GP3218 Δ(bceR-bceS-bceA-bceB)::cat, available in Jörg Stülke's lab
  • MGNA-A167 (ytsC::erm), available at the NBRP B. subtilis, Japan
  • BKE30380 (bceA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTACGCAGTCTCCTTTA, downstream forward: _UP4_CAAGGCGTGTTAGGCGGGGT
  • BKK30380 (bceA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTACGCAGTCTCCTTTA, downstream forward: _UP4_CAAGGCGTGTTAGGCGGGGT

    • Labs working on this gene/protein

  • Susanne Gebhard, Bath UK

    • References

    Reviews

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    Original publications

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