Difference between revisions of "YtsJ"

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(Biological materials)
(Biological materials)
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:''' GP612 (spc), available in [[Stülke]] lab
+
* '''Mutant:'''  
 +
**GP612 (spc), available in [[Stülke]] lab
 +
**GP1143 (spc), available in [[Stülke]] lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 09:05, 30 October 2012

  • Description: malic enzyme, forms a transhydrogenation cycle with MalS for balancing of NADPH

Gene name ytsJ
Synonyms
Essential no
Product NADP-dependent malate dehydrogenase
Function malate utilization
Gene expression levels in SubtiExpress: ytsJ
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 43 kDa, 5.046
Gene length, protein length 1230 bp, 410 aa
Immediate neighbours accD, dnaE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YtsJ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YtsJ expression.png




























Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU29220

Phenotypes of a mutant

Poor growth with malate as single carbon source PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (S)-malate + NADP+ = pyruvate + CO2 + NADPH (according to Swiss-Prot) malate <--> pyruvate
  • Protein family: malic enzymes family (according to Swiss-Prot)
  • Paralogous protein(s): MleA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: Cytoplasm (Homogeneous) PubMed cytoplasm protein PubMed

Database entries

  • Structure: 1WW8 (from Pyrococcus horikoshii, 46% identity, 63% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
    • GP612 (spc), available in Stülke lab
    • GP1143 (spc), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • FLAG-tag construct: GP1131 (spc, based on pGP1331), available in the Stülke lab
  • Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

Your additional remarks

References

Martin Rühl, Dominique Le Coq, Stéphane Aymerich, Uwe Sauer
13C-flux analysis reveals NADPH-balancing transhydrogenation cycles in stationary phase of nitrogen-starving Bacillus subtilis.
J. Biol. Chem.: 2012, 287(33);27959-70
[PubMed:22740702] [WorldCat.org] [DOI] (I p)

Guillaume Lerondel, Thierry Doan, Nicola Zamboni, Uwe Sauer, Stéphane Aymerich
YtsJ has the major physiological role of the four paralogous malic enzyme isoforms in Bacillus subtilis.
J. Bacteriol.: 2006, 188(13);4727-36
[PubMed:16788182] [WorldCat.org] [DOI] (P p)

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

Thierry Doan, Pascale Servant, Shigeo Tojo, Hirotake Yamaguchi, Guillaume Lerondel, Ken-Ichi Yoshida, Yasutaro Fujita, Stéphane Aymerich
The Bacillus subtilis ywkA gene encodes a malic enzyme and its transcription is activated by the YufL/YufM two-component system in response to malate.
Microbiology (Reading, Engl.): 2003, 149(Pt 9);2331-43
[PubMed:12949160] [WorldCat.org] [DOI] (P p)

A Lapidus, N Galleron, A Sorokin, S D Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading, Engl.): 1997, 143 ( Pt 11);3431-41
[PubMed:9387221] [WorldCat.org] [DOI] (P p)