Difference between revisions of "YqhY"

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(Biological materials)
(Biological materials)
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* '''FLAG-tag construct:'''  
 
* '''FLAG-tag construct:'''  
 
** GP1476 (''spc'', based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab
 
** GP1476 (''spc'', based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab
 +
** GP1481 (''spc'', based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab
  
 
* '''Antibody:'''
 
* '''Antibody:'''

Revision as of 11:21, 6 March 2015

  • Description: unknown

Gene name yqhY
Synonyms
Essential no PubMed
Product unknown
Function unknown
Gene expression levels in SubtiExpress: yqhY
Metabolic function and regulation of this protein in SubtiPathways:
yqhY
MW, pI 14 kDa, 4.414
Gene length, protein length 405 bp, 135 aa
Immediate neighbours nusB, accC
Sequences Protein DNA DNA_with_flanks
Genetic context
YqhY context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YqhY expression.png















Categories containing this gene/protein

proteins of unknown function

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU24330

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: asp23 family (according to Swiss-Prot)
  • Paralogous protein(s): YloU

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 127 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 893 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 4905 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 3644 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 4928 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Raphael H Michna, Fabian M Commichau, Dominik Tödter, Christopher P Zschiedrich, Jörg Stülke
SubtiWiki-a database for the model organism Bacillus subtilis that links pathway, interaction and expression information.
Nucleic Acids Res: 2014, 42(Database issue);D692-8
[PubMed:24178028] [WorldCat.org] [DOI] (I p)

Fabian M Commichau, Nico Pietack, Jörg Stülke
Essential genes in Bacillus subtilis: a re-evaluation after ten years.
Mol Biosyst: 2013, 9(6);1068-75
[PubMed:23420519] [WorldCat.org] [DOI] (I p)

Helena B Thomaides, Ella J Davison, Lisa Burston, Hazel Johnson, David R Brown, Alison C Hunt, Jeffery Errington, Lloyd Czaplewski
Essential bacterial functions encoded by gene pairs.
J Bacteriol: 2007, 189(2);591-602
[PubMed:17114254] [WorldCat.org] [DOI] (P p)

P Marini, S J Li, D Gardiol, J E Cronan, D de Mendoza
The genes encoding the biotin carboxyl carrier protein and biotin carboxylase subunits of Bacillus subtilis acetyl coenzyme A carboxylase, the first enzyme of fatty acid synthesis.
J Bacteriol: 1995, 177(23);7003-6
[PubMed:7592499] [WorldCat.org] [DOI] (P p)