Difference between revisions of "YqhY"

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(Biological materials)
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/17114254 PubMed]
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown  
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown  
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= [[Categories]] containing this gene/protein =
= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[proteins of unknown function]]}},
{{SubtiWiki category|[[proteins of unknown function]]}}
{{SubtiWiki category|[[essential genes]]}}
= This gene is a member of the following [[regulons]] =
= This gene is a member of the following [[regulons]] =
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===Phenotypes of a mutant ===
===Phenotypes of a mutant ===
essential [http://www.ncbi.nlm.nih.gov/pubmed/17114254 PubMed]
=== Database entries ===
=== Database entries ===
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* '''Mutant:'''
* '''Mutant:'''
** GP1468 (''yqhY''::''erm''), available in [[Jörg Stülke]]'s lab
** GP1468 (''yqhY''::''erm''), available in [[Jörg Stülke]]'s lab {{PubMed|24178028}}
* '''Expression vector:'''
* '''Expression vector:'''
Line 147: Line 144:
<pubmed>17114254, 7592499 23420519 </pubmed>
<pubmed>17114254, 7592499 23420519 24178028 </pubmed>
[[Category:Protein-coding genes]]
[[Category:Protein-coding genes]]

Revision as of 12:52, 2 November 2013

  • Description: unknown

Gene name yqhY
Essential no
Product unknown
Function unknown
Gene expression levels in SubtiExpress: yqhY
Metabolic function and regulation of this protein in SubtiPathways:
Lipid synthesis
MW, pI 14 kDa, 4.414
Gene length, protein length 405 bp, 135 aa
Immediate neighbours nusB, accC
Sequences Protein DNA DNA_with_flanks
Genetic context
YqhY context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YqhY expression.png

Categories containing this gene/protein

proteins of unknown function

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU24330

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: asp23 family (according to Swiss-Prot)
  • Paralogous protein(s): YloU

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
    • GP1474 (chromosomal yqhY-Strep fusion, aphA3), purification from B. subtilis, for SPINE, available in Jörg Stülke's lab
    • pGP1322 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
    • pGP1325 (N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
  • lacZ fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks


Raphael H Michna, Fabian M Commichau, Dominik Tödter, Christopher P Zschiedrich, Jörg Stülke
SubtiWiki-a database for the model organism Bacillus subtilis that links pathway, interaction and expression information.
Nucleic Acids Res: 2014, 42(Database issue);D692-8
[PubMed:24178028] [WorldCat.org] [DOI] (I p)

Fabian M Commichau, Nico Pietack, Jörg Stülke
Essential genes in Bacillus subtilis: a re-evaluation after ten years.
Mol Biosyst: 2013, 9(6);1068-75
[PubMed:23420519] [WorldCat.org] [DOI] (I p)

Helena B Thomaides, Ella J Davison, Lisa Burston, Hazel Johnson, David R Brown, Alison C Hunt, Jeffery Errington, Lloyd Czaplewski
Essential bacterial functions encoded by gene pairs.
J Bacteriol: 2007, 189(2);591-602
[PubMed:17114254] [WorldCat.org] [DOI] (P p)

P Marini, S J Li, D Gardiol, J E Cronan, D de Mendoza
The genes encoding the biotin carboxyl carrier protein and biotin carboxylase subunits of Bacillus subtilis acetyl coenzyme A carboxylase, the first enzyme of fatty acid synthesis.
J Bacteriol: 1995, 177(23);7003-6
[PubMed:7592499] [WorldCat.org] [DOI] (P p)