Difference between revisions of "YkuN"

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=The gene=
 
=The gene=

Revision as of 08:18, 23 June 2009

  • Description: flavodoxin, binds FMN, replaces ferredoxin under conditions of iorn limitation, probably involved in electron transfer to nitric oxide synthase

Gene name ykuN
Synonyms
Essential no
Product flavodoxin
Function electron transfer
Metabolic function and regulation of this protein in SubtiPathways:
Lys, Thr
MW, pI 17 kDa, 3.827
Gene length, protein length 474 bp, 158 aa
Immediate neighbours ccpC, ykuO
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YkuN context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU14150

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: flavodoxin-like domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • repressed unless the cells enter an iron starvation (Fur) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Rachel J Lawson, Claes von Wachenfeldt, Ihtshamul Haq, John Perkins, Andrew W Munro
Expression and characterization of the two flavodoxin proteins of Bacillus subtilis, YkuN and YkuP: biophysical properties and interactions with cytochrome P450 BioI.
Biochemistry: 2004, 43(39);12390-409
[PubMed:15449930] [WorldCat.org] [DOI] (P p)

Noel Baichoo, Tao Wang, Rick Ye, John D Helmann
Global analysis of the Bacillus subtilis Fur regulon and the iron starvation stimulon.
Mol. Microbiol.: 2002, 45(6);1613-29
[PubMed:12354229] [WorldCat.org] [DOI] (P p)