Difference between revisions of "YabJ"

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=The gene=
 
=The gene=

Revision as of 09:01, 23 June 2009

  • Description: required for activity of PurR

Gene name yabJ
Synonyms
Essential no
Product unknown
Function regulation of purine biosynthesis
Metabolic function and regulation of this protein in SubtiPathways:
Nucleotides (regulation)
MW, pI 13 kDa, 5.134
Gene length, protein length 375 bp, 125 aa
Immediate neighbours purR, spoVG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YabJ context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU00480

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: Ref.3 (according to Swiss-Prot) YjgF family
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions: forms a trimer PubMed
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: negative autoregulation PubMed
    • repressed in the presence of purine nucleotides (PurR) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

S Sinha, P Rappu, S C Lange, P Mäntsälä, H Zalkin, J L Smith
Crystal structure of Bacillus subtilis YabJ, a purine regulatory protein and member of the highly conserved YjgF family.
Proc. Natl. Acad. Sci. U.S.A.: 1999, 96(23);13074-9
[PubMed:10557275] [WorldCat.org] [DOI] (P p)

P Rappu, B S Shin, H Zalkin, P Mäntsälä
A role for a highly conserved protein of unknown function in regulation of Bacillus subtilis purA by the purine repressor.
J. Bacteriol.: 1999, 181(12);3810-5
[PubMed:10368157] [WorldCat.org] (P p)

M Weng, P L Nagy, H Zalkin
Identification of the Bacillus subtilis pur operon repressor.
Proc. Natl. Acad. Sci. U.S.A.: 1995, 92(16);7455-9
[PubMed:7638212] [WorldCat.org] [DOI] (P p)