Difference between revisions of "UreA"

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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 315 bp, 105 aa  
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 315 bp, 105 aa  
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ureB]]'', ''[[csbD]]''
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ureB]]'', ''[[ywzE]]''
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15683&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15683&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''

Revision as of 11:18, 5 December 2012

  • Description: urease (gamma subunit)

Gene name ureA
Essential no
Product urease (gamma subunit)
Function utilization of urea as alternative nitrogen source
Gene expression levels in SubtiExpress: ureA
Interactions involving this protein in SubtInteract: UreA
Metabolic function and regulation of this protein in SubtiPathways:
Purine catabolism, Ammonium/ glutamate, Alternative nitrogen sources
MW, pI 11 kDa, 4.98
Gene length, protein length 315 bp, 105 aa
Immediate neighbours ureB, ywzE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
UreA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
UreA expression.png

Categories containing this gene/protein

utilization of nitrogen sources other than amino acids

This gene is a member of the following regulons

CodY regulon, GlnR regulon, PucR regulon, SigH regulon, TnrA regulon

The gene

Basic information

  • Locus tag: BSU36660

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Urea + H2O = CO2 + 2 NH3 (according to Swiss-Prot)
  • Protein family: urease gamma subunit family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2FVH (from Mycobacterium tuberculosis, 57% identity, 62% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced by nitrogen limitation (GlnR, TnrA) PubMed
    • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks


Boris R Belitsky, Abraham L Sonenshein
Genetic and biochemical analysis of CodY-binding sites in Bacillus subtilis.
J Bacteriol: 2008, 190(4);1224-36
[PubMed:18083814] [WorldCat.org] [DOI] (I p)

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

Jaclyn L Brandenburg, Lewis V Wray, Lars Beier, Hanne Jarmer, Hans H Saxild, Susan H Fisher
Roles of PucR, GlnR, and TnrA in regulating expression of the Bacillus subtilis ure P3 promoter.
J Bacteriol: 2002, 184(21);6060-4
[PubMed:12374841] [WorldCat.org] [DOI] (P p)

L V Wray, A E Ferson, S H Fisher
Expression of the Bacillus subtilis ureABC operon is controlled by multiple regulatory factors including CodY, GlnR, TnrA, and Spo0H.
J Bacteriol: 1997, 179(17);5494-501
[PubMed:9287005] [WorldCat.org] [DOI] (P p)

M R Atkinson, S H Fisher
Identification of genes and gene products whose expression is activated during nitrogen-limited growth in Bacillus subtilis.
J Bacteriol: 1991, 173(1);23-7
[PubMed:1670935] [WorldCat.org] [DOI] (P p)