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  • Description: tryptophan synthase (beta subunit)

Gene name trpB
Essential no
Product tryptophan synthase (beta subunit)
Function biosynthesis of tryptophan
Metabolic function and regulation of this protein in SubtiPathways:
Phe, Tyr, Trp
MW, pI 43 kDa, 5.526
Gene length, protein length 1200 bp, 400 aa
Immediate neighbours trpA, trpF
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
TrpB context.gif
This image was kindly provided by SubtiList

The gene

Basic information

  • Locus tag: BSU22640

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + glyceraldehyde 3-phosphate + H2O (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism: TRAP: binding to the mRNA in the presence of tryptophan, this results in transcription termination PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks


Gintaras Deikus, David H Bechhofer
Initiation of decay of Bacillus subtilis trp leader RNA.
J. Biol. Chem.: 2007, 282(28);20238-44
[PubMed:17507374] [] [DOI] (P p)

Gintaras Deikus, Paul Babitzke, David H Bechhofer
Recycling of a regulatory protein by degradation of the RNA to which it binds.
Proc. Natl. Acad. Sci. U.S.A.: 2004, 101(9);2747-51
[PubMed:14976255] [] [DOI] (P p)

J Otridge, P Gollnick
MtrB from Bacillus subtilis binds specifically to trp leader RNA in a tryptophan-dependent manner.
Proc. Natl. Acad. Sci. U.S.A.: 1993, 90(1);128-32
[PubMed:8419914] [] [DOI] (P p)

P Babitzke, P Gollnick, C Yanofsky
The mtrAB operon of Bacillus subtilis encodes GTP cyclohydrolase I (MtrA), an enzyme involved in folic acid biosynthesis, and MtrB, a regulator of tryptophan biosynthesis.
J. Bacteriol.: 1992, 174(7);2059-64
[PubMed:1551827] [] [DOI] (P p)

H Shimotsu, M I Kuroda, C Yanofsky, D J Henner
Novel form of transcription attenuation regulates expression the Bacillus subtilis tryptophan operon.
J. Bacteriol.: 1986, 166(2);461-71
[PubMed:2422155] [] [DOI] (P p)

D J Henner, L Band, H Shimotsu
Nucleotide sequence of the Bacillus subtilis tryptophan operon.
Gene: 1985, 34(2-3);169-77
[PubMed:3924737] [] [DOI] (P p)

H Shimotsu, D J Henner
Characterization of the Bacillus subtilis tryptophan promoter region.
Proc. Natl. Acad. Sci. U.S.A.: 1984, 81(20);6315-9
[PubMed:6436812] [] [DOI] (P p)