SufS

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  • Description: cysteine desulfurase, cysteine:SufU sulfuryl transferase

Gene name sufS
Synonyms yurW, csd
Essential yes PubMed
Product cysteine desulfurase
Function formation of iron-sulfur clusters in proteins
Gene expression levels in SubtiExpress: sufS
Interactions involving this protein in SubtInteract: SufS
Metabolic function and regulation of this protein in SubtiPathways:
SufS
MW, pI 44 kDa, 5.199
Gene length, protein length 1218 bp, 406 aa
Immediate neighbours sufU, sufD
Sequences Protein DNA DNA_with_flanks
Genetic context
Csd context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SufS expression.png















Categories containing this gene/protein

iron metabolism, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU32690

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: transfer of iron to SufU PubMed
  • Protein family: Csd subfamily (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity: interaction with SufU enhances the activity of SufS 40-fold PubMed

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 1121 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 1680 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 3779 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 2246 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 2854 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Katherine A Black, Patricia C Dos Santos
Shared-intermediates in the biosynthesis of thio-cofactors: Mechanism and functions of cysteine desulfurases and sulfur acceptors.
Biochim. Biophys. Acta: 2015, 1853(6);1470-80
[PubMed:25447671] [WorldCat.org] [DOI] (P p)

Original publications