Difference between revisions of "SpoVS"

From SubtiWiki
Jump to: navigation, search
(Biological materials)
Line 123: Line 123:
 
=Biological materials =
 
=Biological materials =
  
* '''Mutant:'''
+
* '''Mutant:''' GP1601 (cat), available in [[Stülke]] lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 09:39, 28 January 2014

  • Description: required for dehydratation of the spore core and assembly of the coat, mutation increases SigD-dependent gene expression, might act via SinR

Gene name spoVS
Synonyms
Essential no
Product unknown
Function spore coat assembly, spore core dehydratation
Gene expression levels in SubtiExpress: spoVS
MW, pI 8 kDa, 6.475
Gene length, protein length 258 bp, 86 aa
Immediate neighbours ymdB, tdh
Sequences Protein DNA DNA_with_flanks
Genetic context
SpoVS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SpoVS expression.png
























Categories containing this gene/protein

sporulation/ other

This gene is a member of the following regulons

SigH regulon

The gene

Basic information

  • Locus tag: BSU16980

Phenotypes of a mutant

increased SigD-dependent gene expression, this might occur via SinR PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed at the onset of stationary phase (SigH) PubMed
  • Regulatory mechanism:
  • Additional information:
    • The mRNA has a long 5' leader region. This may indicate RNA-based regulation PubMed

Biological materials

  • Mutant: GP1601 (cat), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res.: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Daniel J Rigden, Michael Y Galperin
Sequence analysis of GerM and SpoVS, uncharacterized bacterial 'sporulation' proteins with widespread phylogenetic distribution.
Bioinformatics: 2008, 24(16);1793-7
[PubMed:18562273] [WorldCat.org] [DOI] (I p)

Ana R Perez, Angelica Abanes-De Mello, Kit Pogliano
Suppression of engulfment defects in bacillus subtilis by elevated expression of the motility regulon.
J. Bacteriol.: 2006, 188(3);1159-64
[PubMed:16428420] [WorldCat.org] [DOI] (P p)

O Resnekov, A Driks, R Losick
Identification and characterization of sporulation gene spoVS from Bacillus subtilis.
J. Bacteriol.: 1995, 177(19);5628-35
[PubMed:7559352] [WorldCat.org] [DOI] (P p)